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Anti-MRP1 antibody [EPR22841-78] (ab260038) is a rabbit monoclonal antibody that is used to detect MRP1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P. Suitable for Human, Mouse samples.



- Specificity confirmed with MRP1 knockout cell line validation

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Images

Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (AB260038), expandable thumbnail
  • Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (AB260038), expandable thumbnail
  • Western blot - Anti-MRP1 antibody [EPR22841-78] (AB260038), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MRP1 antibody [EPR22841-78] (AB260038), expandable thumbnail
  • Western blot - Anti-MRP1 antibody [EPR22841-78] (AB260038), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Tested
Tested
Not recommended
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/600
Notes

-

Species
Human
Dilution info
1/600
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Mediates export of organic anions and drugs from the cytoplasm (PubMed:10064732, PubMed:11114332, PubMed:16230346, PubMed:7961706, PubMed:9281595). Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics (PubMed:10064732, PubMed:11114332, PubMed:16230346, PubMed:7961706, PubMed:9281595). Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export (PubMed:9281595). Hydrolyzes ATP with low efficiency (PubMed:16230346). Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation (PubMed:17050692). Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells (By similarity). Mediates ATP-dependent, GSH-independent cyclic GMP-AMP (cGAMP) export (PubMed:36070769). Thus, by limiting intracellular cGAMP concentrations negatively regulates the cGAS-STING pathway (PubMed:36070769).

Alternative names

MRP, MRP1, ABCC1, Multidrug resistance-associated protein 1, ATP-binding cassette sub-family C member 1, Glutathione-S-conjugate-translocating ATPase ABCC1, Leukotriene C(4) transporter, LTC4 transporter

Recommended products

Anti-MRP1 antibody [EPR22841-78] (ab260038) is a rabbit monoclonal antibody that is used to detect MRP1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P. Suitable for Human, Mouse samples.



- Specificity confirmed with MRP1 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22841-78
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-MRP1 antibody [EPR22841-78] (ab260038) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), in Human, Mouse samples.

What is the molecular weight of MRP1?


Anti-MRP1 [EPR22841-78] (ab260038) specifically detects a band for MRP1 (UniProt: P33527) at a molecular weight of 171kDa.

Recommended positive controls


WB: A549, HepG2, K-562, Human lung cancer, Human lung, NIH/3T3, Mouse liver and Mouse stomach lysates. IHC-P: Human breast carcinoma, Human gastric adenocarcinoma, Human lung carcinoma and the paired adjacent lung and Mouse stomach tissues. Flow Cyt (intra): HeLa, A549 and NIH/3T3 cells. IP: A549 and NIH/3T3 cells.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed


The specificity of Anti-MRP1 antibody [EPR22841-78] (ab260038) has been confirmed by Flow Cytometry testing in ABCC1 Knockout HeLa cell line, Human ABCC1 (MRP1) knockout HeLa cell line ab265256.



Other related products


We have a range of other formats of antibody clone [EPR22841-78] also available for your convenience:
ab260038, Carrier free - Anti-MRP1 antibody [EPR22841-78] - BSA and Azide free ab263865



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MRP1 also known as ABCC1 is a transporter protein with a mass of approximately 190 kDa. This protein belongs to the ATP-binding cassette (ABC) transporter family. MRP1 actively exports a variety of substrates from cells by hydrolyzing ATP to ADP and inorganic phosphate. You can find MRP1 expressed in many tissues including the lung testis and kidney. It helps in cellular detoxification by exporting organic anions and other conjugated metabolites.

Biological function summary

This protein functions as an important determinant of multidrug resistance in cancer treatment. MRP1 can form part of a larger protein complex that includes other transporters like MRP2. Through its activity MRP1 protects tissues from toxic substances by transporting them out of the cell. Its role in transporting glutathione-conjugated compounds highlights its importance in cellular defense mechanisms against oxidative stress and xenobiotics.

Pathways

MRP1 participates in the glutathione metabolism and the xenobiotic efflux pathways. Both pathways involve cellular detoxification and the accumulation of anomalies can cause harmful effects in the body. MRP1 works with proteins such as GSTP1 which conjugates toxic substances with glutathione preparing them for export by MRP1. This coordination ensures efficient detoxification and protection of cells from damage.

Associated diseases and disorders

MRP1 has associations with several conditions particularly drug resistance in various cancers and chronic obstructive pulmonary disease (COPD). In cancer MRP1 overexpression often results in reduced treatment efficacy due to chemotherapy drugs being expelled from the cell helping to resist their cytotoxic effects. Research indicates a connection between MRP1 and P-glycoprotein (ABCB1) in cancer drug resistance pointing to a broader resistive mechanism in which multiple transporters are involved.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (ab260038), expandable thumbnail

    Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (ab260038)

    MRP1 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug with ab260038 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution. Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab260038 IP in A549 (human lung carcinoma epithelial cell) whole cell lysate, boiled 10ug

    Lane 3: A549 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

    All lanes: Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (ab260038)

    Predicted band size: 171 kDa

    Observed band size: 190 kDa

  • Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (ab260038), expandable thumbnail

    Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (ab260038)

    MRP1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug with ab260038 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution. Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug

    Lane 2: ab260038 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate, boiled 10ug

    Lane 3: NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 75 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

    All lanes: Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (ab260038)

    Predicted band size: 171 kDa

    Observed band size: 190 kDa

  • Western blot - Anti-MRP1 antibody [EPR22841-78] (ab260038), expandable thumbnail

    Western blot - Anti-MRP1 antibody [EPR22841-78] (ab260038)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 27634913).

    Weakly expressing control: K-562, lung, liver, HEK293 (PMID: 18347152, 25880778,11059771, 29615646, 26853103).

    Samples (except lane1) are non-boiled as boiling may cause protein aggregates. The Lane 1 lysate was boiled on 100℃ water.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-MRP1 antibody [EPR22841-78] (ab260038) at 1/1000 dilution

    Lanes 1 - 2: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lane 4: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 5: Human lung cancer lysate at 20 µg

    Secondary

    Lanes 1, 4 and 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 2 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/25000 dilution

    Predicted band size: 171 kDa

    Observed band size: 120-250 kDa

  • Flow Cytometry (Intracellular) - Anti-MRP1 antibody [EPR22841-78] (ab260038), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MRP1 antibody [EPR22841-78] (ab260038)

    Intracellular Flow Cytometry overlay histogram showing wild-type HeLa (green line) and ABCC1 knockout HeLa cells (Human ABCC1 (MRP1) knockout HeLa cell line ab265256). The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used at 1/2000 for 30 min at 22°C.) stained with ab260038 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab260038) (1x106 in 100μl at 0.2 μg/ml) for 30 min at 22°C.

    Isotype control antibody was Rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line ABCC1 knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Western blot - Anti-MRP1 antibody [EPR22841-78] (ab260038), expandable thumbnail

    Western blot - Anti-MRP1 antibody [EPR22841-78] (ab260038)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 27634913).

    Weakly expressing control: K-562, lung, liver, HEK293 (PMID: 18347152, 25880778,11059771, 29615646, 26853103).

    All lysates were unboiled.

    Exposure time: Lanes 1-3: 10 seconds; Lanes 4-8: 3 minutes.

    All lanes: Western blot - Anti-MRP1 antibody [EPR22841-78] (ab260038) at 1/1000 dilution

    Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 4: Human lung cancer lysate at 20 µg

    Lane 5: Human lung lysate at 20 µg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

    Lane 7: Mouse liver lysate at 10 µg

    Lane 8: Mouse stomach lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 171 kDa

    Observed band size: 190 kDa

  • Flow Cytometry (Intracellular) - Anti-MRP1 antibody [EPR22841-78] (ab260038), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MRP1 antibody [EPR22841-78] (ab260038)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell, Left) / A549 (human lung carcinoma epithelial cell, Right) cells labelling MRP1 with ab260038 at 1/600 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

  • Flow Cytometry (Intracellular) - Anti-MRP1 antibody [EPR22841-78] (ab260038), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MRP1 antibody [EPR22841-78] (ab260038)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling MRP1 with ab260038 at 1/600 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260038).

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