Anti-MRP1 antibody [EPR4658(2)] - C-terminal

• WB

Supplier Data

Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (AB180960)

Lanes 1 - 2 : Merged signal (red and green). Green - ab180960 observed at 170 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab180960 was shown to specifically react with ABCC1 in wild-type A549 cells as signal was lost in ABCC1 knockout cell line ab261871 (knockout cell lysate ab261680). Wild-type and ABCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab180960 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ABCC1 (MRP1) knockout A549 cell lysate (<a href='/en-us/products/cell-lysates/human-abcc1-mrp1-knockout-a549-cell-lysate-ab261680'>ab261680</a>) at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Lanes 1 - 2:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 171 kDa

Observed band size: 171 kDa,130 kDa

false

• WB

Lab

Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (AB180960)

Blocking and dilution buffer : 5% NFDM/TBST.

If there is no ideal result on boiled sample, we suggest not to boil sample before loading onto the gel.

All lanes:

Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (boiled) at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 171 kDa

false

Exposure time: 20s

• WB

Supplier Data

Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (AB180960)

All lanes:

Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/5000 dilution

All lanes:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 171 kDa

false