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AB24102

Anti-MRP1 antibody [MRPm5]

4

(3 Reviews)

|

(67 Publications)

Mouse Monoclonal MRP1 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 67 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCC1 aa 950 to C-terminus.

View Alternative Names

MRP, MRP1, ABCC1, Multidrug resistance-associated protein 1, ATP-binding cassette sub-family C member 1, Glutathione-S-conjugate-translocating ATPase ABCC1, Leukotriene C(4) transporter, LTC4 transporter

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MRPm5

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB

applications

Immunogen

Recombinant Fragment Protein within Human ABCC1 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P33527

Epitope

MRP1 antibody reacts with an internal epitope of MRP1.

Specificity

MRPm5 reacts with an internal epitope of MRP1, a 180-195 kD transmembrane transporter protein overexpressed in various
human non-P-glycoprotein MDR tumor cell lines.

MRPm5 does not cross-react with the human MDR1 and MDR3 gene products.

Reactivity data

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRP1 also known as ABCC1 is a transporter protein with a mass of approximately 190 kDa. This protein belongs to the ATP-binding cassette (ABC) transporter family. MRP1 actively exports a variety of substrates from cells by hydrolyzing ATP to ADP and inorganic phosphate. You can find MRP1 expressed in many tissues including the lung testis and kidney. It helps in cellular detoxification by exporting organic anions and other conjugated metabolites.
Biological function summary

This protein functions as an important determinant of multidrug resistance in cancer treatment. MRP1 can form part of a larger protein complex that includes other transporters like MRP2. Through its activity MRP1 protects tissues from toxic substances by transporting them out of the cell. Its role in transporting glutathione-conjugated compounds highlights its importance in cellular defense mechanisms against oxidative stress and xenobiotics.

Pathways

MRP1 participates in the glutathione metabolism and the xenobiotic efflux pathways. Both pathways involve cellular detoxification and the accumulation of anomalies can cause harmful effects in the body. MRP1 works with proteins such as GSTP1 which conjugates toxic substances with glutathione preparing them for export by MRP1. This coordination ensures efficient detoxification and protection of cells from damage.

MRP1 has associations with several conditions particularly drug resistance in various cancers and chronic obstructive pulmonary disease (COPD). In cancer MRP1 overexpression often results in reduced treatment efficacy due to chemotherapy drugs being expelled from the cell helping to resist their cytotoxic effects. Research indicates a connection between MRP1 and P-glycoprotein (ABCB1) in cancer drug resistance pointing to a broader resistive mechanism in which multiple transporters are involved.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Mediates export of organic anions and drugs from the cytoplasm (PubMed : 10064732, PubMed : 11114332, PubMed : 16230346, PubMed : 7961706, PubMed : 9281595). Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics (PubMed : 10064732, PubMed : 11114332, PubMed : 16230346, PubMed : 7961706, PubMed : 9281595). Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export (PubMed : 9281595). Hydrolyzes ATP with low efficiency (PubMed : 16230346). Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation (PubMed : 17050692). Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthesizing cells (By similarity). Mediates ATP-dependent, GSH-independent cyclic GMP-AMP (cGAMP) export (PubMed : 36070769). Thus, by limiting intracellular cGAMP concentrations negatively regulates the cGAS-STING pathway (PubMed : 36070769). Exports S-geranylgeranyl-glutathione (GGG) in lymphoid cells and stromal compartments of lymphoid organs. ABCC1 (via extracellular transport) with GGT5 (via GGG catabolism) establish GGG gradients within lymphoid tissues to position P2RY8-positive lymphocytes at germinal centers in lymphoid follicles and restrict their chemotactic transmigration from blood vessels to the bone marrow parenchyma (By similarity). Mediates basolateral export of GSH-conjugated R- and S-prostaglandin A2 diastereomers in polarized epithelial cells (PubMed : 9426231).
See full target information ABCC1

Publications (67)

Recent publications for all applications. Explore the full list and refine your search

Basic and clinical neuroscience 14:615-630 PubMed38628830

2024

Expressional Study of Permeability Glycoprotein and Multidrug Resistance Protein 1 in Drug-resistant Mesial Temporal Lobe Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Mandeep Kaur,Tulika Gupta,Mili Gupta,Navneet Singla,Parampreet S Kharbanda,Yogender Singh Bansal,Daisy Sahni,Bishan Das Radotra,Sunil Kumar Gupta

Fluids and barriers of the CNS 21:5 PubMed38200564

2024

Cocaine regulates antiretroviral therapy CNS access through pregnane-x receptor-mediated drug transporter and metabolizing enzyme modulation at the blood brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Rodnie Colón Ortiz,Stephen Knerler,Lisa B Fridman,Alicia Mercado,Amira-Storm Price,Jose J Rosado-Franco,Hannah Wilkins,Bianca R Flores,Benjamin C Orsburn,Dionna W Williams

Nature communications 14:5709 PubMed37726279

2023

ABCC1 and glutathione metabolism limit the efficacy of BCL-2 inhibitors in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Ebner,Johannes Schmoellerl,Martin Piontek,Gabriele Manhart,Selina Troester,Bing Z Carter,Heidi Neubauer,Richard Moriggl,Gergely Szakács,Johannes Zuber,Thomas Köcher,Michael Andreeff,Wolfgang R Sperr,Peter Valent,Florian Grebien

Cancers 15: PubMed37627134

2023

Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Danijela Mandić,Lana Nežić,Ljiljana Amdžić,Nataša Vojinović,Radoslav Gajanin,Miroslav Popović,Jugoslav Đeri,Milena Todorović Balint,Jelena Dumanović,Zoran Milovanović,Jelica Grujić-Milanović,Ranko Škrbić,Vesna Jaćević

Journal of Cancer 14:1515-1530 PubMed37325046

2023

Proline-rich acidic protein 1 upregulates mitotic arrest deficient 1 to promote cisplatin-resistance of colorectal carcinoma by restraining mitotic checkpoint complex assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Jintian Song,Yigui Chen,Hui Yu,Liang Zheng,Yi Wang,Dan Li

Cancer science 114:3027-3040 PubMed37190912

2023

Plasma miR-1254 as a predictive biomarker of chemosensitivity and a target of nucleic acid therapy in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Takashima,Shuhei Komatsu,Takuma Ohashi,Jun Kiuchi,Keiji Nishibeppu,Hajime Kamiya,Hiroshi Arakawa,Ryo Ishida,Hiroki Shimizu,Tomohiro Arita,Hirotaka Konishi,Atsushi Shiozaki,Takeshi Kubota,Hitoshi Fujiwara,Eigo Otsuji

ALTEX 40:103-116 PubMed35648122

2022

Beyond pharmaceuticals: Fit-for-purpose new approach methodologies for environmental cardiotoxicity testing.

Applications

Unspecified application

Species

Unspecified reactive species

Mark C Daley,Ulrike Mende,Bum-Rak Choi,Patrick D McMullen,Kareen L K Coulombe

ALTEX 40:34–52 PubMed35575642

2022

Development of a network of carcinogenicity adverse outcome pathways and its employment as an evidence framework for safety assessment

Applications

Unspecified application

Species

Unspecified reactive species

Alex N Cayley,Robert S Foster,Emma Hill,Steven Kane,Grace Kocks,Alun Myden,Daniel Newman,Susanne A Stalford,Jonathan D Vessey,Reza Zarei,Antonio Anax F De Oliveira

ALTEX 40:61-82 PubMed35536601

2022

A microfluidic thyroid-liver platform to assess chemical safety in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Kühnlenz,Diana Karwelat,Thomas Steger-Hartmann,Marian Raschke,Sophie Bauer,Özlem Vural,Uwe Marx,Helen Tinwell,Remi Bars

Nature communications 13:2083 PubMed35440587

2022

Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes.

Applications

Unspecified application

Species

Unspecified reactive species

Norman L Lehman,Nathalie Spassky,Müge Sak,Amy Webb,Cory T Zumbar,Aisulu Usubalieva,Khaled J Alkhateeb,Joseph P McElroy,Kirsteen H Maclean,Paolo Fadda,Tom Liu,Vineela Gangalapudi,Jamie Carver,Zied Abdullaev,Cynthia Timmers,John R Parker,Christopher R Pierson,Bret C Mobley,Murat Gokden,Eyas M Hattab,Timothy Parrett,Ralph X Cooke,Trang D Lehman,Stefan Costinean,Anil Parwani,Brian J Williams,Randy L Jensen,Kenneth Aldape,Akshitkumar M Mistry
View all publications

Product promise

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