Anti-MRP2 antibody [EPR10998]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MRP2 antibody [EPR10998] (AB172630)

Intracellular flow cytometric analysis of permeabilized A549 cells usingab172630 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10998] (AB172630)

Immunohistochemistry analysis of Paraffin-embedded human kidney tissue sections labeling MRP2 with ab172630 at 10000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on human kidney. The section was incubated with ab172630 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10998] (AB172630)

Immunohistochemistry analysis of Paraffin-embedded human tonsil tissue sections labeling MRP2 with ab172630 at 10000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Negative control : no staining on the human tonsil. The section was incubated with ab172630 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10998] (AB172630)

Immunohistochemistry analysis of Paraffin-embedded human liver tissue sections labeling MRP2 with ab172630 at 10000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the bile ducts in human liver. The section was incubated with ab172630 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRP2 antibody [EPR10998] (AB172630)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling MRP2 with purified ab172630 at 1 : 100 dilution (7.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRP2 antibody [EPR10998] (AB172630)

Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling MRP2 with ab172630 at a 1/250 dilution.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MRP2 antibody [EPR10998] (AB172630)

Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling MRP2 with purified ab172630 at 1/70 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10998] (AB172630)

Immunohistochemistry analysis of Paraffin-embedded mouse liver tissue sections labeling MRP2 with ab172630 at 10000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the bile ducts in mouse liver. The section was incubated with ab172630 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• WB

Lab

Western blot - Anti-MRP2 antibody [EPR10998] (AB172630)

The multiple bands are consistent with what has been described in PMID : 18832477

All lanes:

Western blot - Anti-MRP2 antibody [EPR10998] (ab172630) at 1/2000 dilution

All lanes:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 174 kDa

Observed band size: >190 kDa

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• WB

Supplier Data

Western blot - Anti-MRP2 antibody [EPR10998] (AB172630)

All lanes:

Western blot - Anti-MRP2 antibody [EPR10998] (ab172630) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

HepG2 lysate at 10 µg

Lane 3:

A549 lysate at 10 µg

Predicted band size: 174 kDa

false

• WB

Lab

Western blot - Anti-MRP2 antibody [EPR10998] (AB172630)

Lanes 1 - 4 : Merged signal (red and green). Green - ab172630 observed at 210 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab172630 was shown to recognize MRP2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab172630 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MRP2 antibody [EPR10998] (ab172630) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

ABCC2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ABCC2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-abcc2-knockout-a549-cell-line-ab261855'>ab261855</a>)

Lane 3:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 174 kDa

false

• WB

CiteAb

Western blot - Anti-MRP2 antibody [EPR10998] (AB172630)

MRP2 western blot using anti-MRP2 antibody [EPR10998] ab172630. Publication image and figure legend from Wu, L., Li, Y., et al., 2020, Orphanet J Rare Dis, PubMed 32183854.

ab172630 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab172630 please see the product overview.

The MRP2 p.G693R mutant showed decreased expression and mislocalization in three cell lines in vitro. a Western blot demonstrated significantly decreased expression of MRP2 in three cell lines expressing the p.G693R mutant compared with those expressing wild-type MRP2. **p < 0.05. b Immunofluorescence assay showed the mislocalization of the MRP2 p.G693R mutant, which was predominantly retained in the cytoplasm rather than the cell surface

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