Anti-MRP2 antibody [EPR10998] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRP2 antibody [EPR10998] - BSA and Azide free (AB240169)

Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling MRP2 with ab172630 at a 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172630).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MRP2 antibody [EPR10998] - BSA and Azide free (AB240169)

Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling MRP2 with purified ab172630 at 1/70 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MRP2 antibody [EPR10998] - BSA and Azide free (AB240169)

Intracellular flow cytometric analysis of permeabilized A549 cells using ab172630 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172630).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRP2 antibody [EPR10998] - BSA and Azide free (AB240169)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling MRP2 with purified ab172630 at 1 : 100 dilution (7.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10998] - BSA and Azide free (AB240169)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172630).  Immunohistochemistry analysis of Paraffin-embedded human liver tissue sections labeling MRP2 with ab172630 at 10000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the bile ducts in human liver. The section was incubated with ab172630 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Confocal image showing Mitochondria staining in Jurkat cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10998] - BSA and Azide free (AB240169)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172630).  Immunohistochemistry analysis of Paraffin-embedded human kidney tissue sections labeling MRP2 with ab172630 at 10000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on human kidney. The section was incubated with ab172630 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10998] - BSA and Azide free (AB240169)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172630).  Immunohistochemistry analysis of Paraffin-embedded human tonsil tissue sections labeling MRP2 with ab172630 at 10000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Negative control : no staining on the human tonsil. The section was incubated with ab172630 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10998] - BSA and Azide free (AB240169)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172630).  Immunohistochemistry analysis of Paraffin-embedded mouse liver tissue sections labeling MRP2 with ab172630 at 10000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on the bile ducts in mouse liver. The section was incubated with ab172630 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.