Anti-MRP2 antibody [M2 III-6]

• WB

Lab

Western blot - Anti-MRP2 antibody [M2 III-6] (AB3373)

Lanes 1 - 3 : Merged signal (red and green). Green - ab3373 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.

ab3373 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab3373 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/20 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

ABCC2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ABCC2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-abcc2-knockout-a549-cell-line-ab261855'>ab261855</a>)

Lane 3:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 174 kDa

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• WB

AbReview57224****

Western blot - Anti-MRP2 antibody [M2 III-6] (AB3373)

All lanes:

Western blot - Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/500 dilution

All lanes:

WIF-B cells, the hepatoma-derived hybrid cell line at 30 µg with 5% milk in PBST

Secondary

All lanes:

HRP conjugated Donkey anti-Mouse at 1/10000 dilution

Predicted band size: 174 kDa

Observed band size: 130-250 kDa

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Exposure time: 20min

This image is courtesy of an anonymous Abreview

• WB

CiteAb

Western blot - Anti-MRP2 antibody [M2 III-6] (AB3373)

MRP2 western blot using anti-MRP2 antibody [M2 III-6] ab3373. Publication image and figure legend from Topalis, D., Nogueira, T. C., et al., 2016, Oncotarget, PubMed 26824416.

ab3373 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab3373 please see the product overview.

Differential expression of several ABC and SLC transportersWestern blot detection was performed to measure the levels of OAT1 and BCRP (A), MRP2 (B), and P-gp (C). Quantification of each band was done and standardized using actin as housekeeping gene (D).

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