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AB226804

Anti-MRP3 antibody

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(1 Publication)

Rabbit Polyclonal MRP3 antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ABCC3.

View Alternative Names

CMOAT2, MLP2, MRP3, ABCC3, ATP-binding cassette sub-family C member 3, Canalicular multispecific organic anion transporter 2, Multi-specific organic anion transporter D, Multidrug resistance-associated protein 3, MOAT-D

3 Images
Western blot - Anti-MRP3 antibody (AB226804)
  • WB

Supplier Data

Western blot - Anti-MRP3 antibody (AB226804)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-MRP3 antibody (ab226804) at 1/500 dilution

Lane 1:

HCT 116 (Human colorectal carcinoma cell line) whole cell extract at 30 µg

Lane 2:

HCT 116 (Human colorectal carcinoma cell line) membrane extract at 30 µg

Predicted band size: 169 kDa

false

Western blot - Anti-MRP3 antibody (AB226804)
  • WB

Supplier Data

Western blot - Anti-MRP3 antibody (AB226804)

All lanes:

Western blot - Anti-MRP3 antibody (ab226804) at 1/500 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 169 kDa

false

Western blot - Anti-MRP3 antibody (AB226804)
  • WB

Supplier Data

Western blot - Anti-MRP3 antibody (AB226804)

All lanes:

Western blot - Anti-MRP3 antibody (ab226804) at 1/500 dilution

All lanes:

Rat liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 169 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ABCC3. The exact immunogen used to generate this antibody is proprietary information.

O15438

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRP3 also known as ABCC3 is a membrane-associated protein that plays an important role in transmembrane transport. This protein weighs approximately 170 kDa and is part of the ATP-binding cassette (ABC) transporter superfamily. MRP3 primarily localizes in the liver intestine and adrenal gland with presence also observed in the kidneys and pancreas. Its structural formation includes multiple transmembrane domains which facilitate the export of diverse molecules including endogenous substances and xenobiotics.
Biological function summary

MRP3 functions in the transport of organic anions across cellular membranes. It does not operate in isolation often interacting within a network of transporters to move glucuronidated sulfated and other conjugated organic anions from the cells. This protein plays a significant role in the body's detoxification processes where it assists in duly removing harmful metabolites and drugs. The MRP3's ability to transport bile acids and conjugates suggests it influences lipid metabolism as well.

Pathways

MRP3 is part of the glutathione and bile acid recycling pathways. Through these pathways MRP3 serves to recycle and transport surplus bile acids from the liver to the blood. This function connects MRP3 with other proteins like BSEP and MRP2 which also deal in bile acid transport. By regulating these pathways MRP3 maintains and controls the homeostasis of essential molecules within the cellular environment.

MRP3 links to conditions such as cholestasis and liver disease. Its dysregulation can result in impaired detoxification and transport processes exacerbating these conditions. An association exists between MRP3 and MRP2 where mutations or overexpression in these related proteins may impact disease progression and severity. Researchers study MRP3 to better understand its role in the pathology of these diseases seeking therapeutic insights and interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent transporter of the ATP-binding cassette (ABC) family that binds and hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants and endogenous compound across cell membranes (PubMed : 10359813, PubMed : 11581266, PubMed : 15083066). Transports glucuronide conjugates such as bilirubin diglucuronide, estradiol-17-beta-o-glucuronide and GSH conjugates such as leukotriene C4 (LTC4) (PubMed : 11581266, PubMed : 15083066). Transports also various bile salts (taurocholate, glycocholate, taurochenodeoxycholate-3-sulfate, taurolithocholate- 3-sulfate) (By similarity). Does not contribute substantially to bile salt physiology but provides an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity). May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (Probable). Can confer resistance to various anticancer drugs, methotrexate, tenoposide and etoposide, by decreasing accumulation of these drugs in cells (PubMed : 10359813, PubMed : 11581266).
See full target information ABCC3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 44:403-418 PubMed31103629

2019

HNF1A inhibition induces the resistance of pancreatic cancer cells to gemcitabine by targeting ABCB1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Lu,Dongni Xu,Jintao Peng,Zhaofan Luo,Chujie Chen,Yuqing Chen,Huimou Chen,Minghui Zheng,Peihong Yin,Zhi Wang
View all publications
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