AB3375

Anti-MRP3 antibody [M3II-9]

Name: Anti-MRP3 antibody [M3II-9] (ab3375) | Abcam
Brand: Abcam Limited
SKU: AB3375
Price: 565 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(18 Publications)

Mouse Monoclonal MRP3 antibody. Suitable for WB and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCC3 aa 800-950.

View Alternative Names

CMOAT2, MLP2, MRP3, ABCC3, ATP-binding cassette sub-family C member 3, Canalicular multispecific organic anion transporter 2, Multi-specific organic anion transporter D, Multidrug resistance-associated protein 3, MOAT-D

1 Images

Functional Studies - Anti-MRP3 antibody [M3II-9] (AB3375)

FuncS

Unknown

Functional Studies - Anti-MRP3 antibody [M3II-9] (AB3375)

HepG2 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of clotrimazole (ab120744) in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M3II-9

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ABCC3 aa 800-950. The exact immunogen used to generate this antibody is proprietary information.

O15438

Epitope

This antibody detects with an internal epitope of the membrane transport protein of MRP3

Specificity

This antibody does not cross react with MDR1, MRP1, MRP2 or MRP5 gene products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20 - 1/50", "WB-species-notes": "<p></p>" } } }

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Product details

The rat species was added as a result of positive Abreviews and publication PMID 22112382. In response to few recent complaints, we have decided to remove this species and no longer guarantee it. Please contact technical@abcam.com for any inquiry.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 Constituents: 0.7% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRP3 also known as ABCC3 is a membrane-associated protein that plays an important role in transmembrane transport. This protein weighs approximately 170 kDa and is part of the ATP-binding cassette (ABC) transporter superfamily. MRP3 primarily localizes in the liver intestine and adrenal gland with presence also observed in the kidneys and pancreas. Its structural formation includes multiple transmembrane domains which facilitate the export of diverse molecules including endogenous substances and xenobiotics.

Biological function summary

MRP3 functions in the transport of organic anions across cellular membranes. It does not operate in isolation often interacting within a network of transporters to move glucuronidated sulfated and other conjugated organic anions from the cells. This protein plays a significant role in the body's detoxification processes where it assists in duly removing harmful metabolites and drugs. The MRP3's ability to transport bile acids and conjugates suggests it influences lipid metabolism as well.

Pathways

MRP3 is part of the glutathione and bile acid recycling pathways. Through these pathways MRP3 serves to recycle and transport surplus bile acids from the liver to the blood. This function connects MRP3 with other proteins like BSEP and MRP2 which also deal in bile acid transport. By regulating these pathways MRP3 maintains and controls the homeostasis of essential molecules within the cellular environment.

MRP3 links to conditions such as cholestasis and liver disease. Its dysregulation can result in impaired detoxification and transport processes exacerbating these conditions. An association exists between MRP3 and MRP2 where mutations or overexpression in these related proteins may impact disease progression and severity. Researchers study MRP3 to better understand its role in the pathology of these diseases seeking therapeutic insights and interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

ATP-dependent transporter of the ATP-binding cassette (ABC) family that binds and hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants and endogenous compound across cell membranes (PubMed : 10359813, PubMed : 11581266, PubMed : 15083066). Transports glucuronide conjugates such as bilirubin diglucuronide, estradiol-17-beta-o-glucuronide and GSH conjugates such as leukotriene C4 (LTC4) (PubMed : 11581266, PubMed : 15083066). Transports also various bile salts (taurocholate, glycocholate, taurochenodeoxycholate-3-sulfate, taurolithocholate- 3-sulfate) (By similarity). Does not contribute substantially to bile salt physiology but provides an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity). May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (Probable). Can confer resistance to various anticancer drugs, methotrexate, tenoposide and etoposide, by decreasing accumulation of these drugs in cells (PubMed : 10359813, PubMed : 11581266).

See full target information ABCC3

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 115:1778-1790 PubMed38566304

2024

Downregulation of ABCC3 activates MAPK signaling through accumulation of deoxycholic acid in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yukihiro Sato,Minoru Kobayashi,Masahiro Ohira,Ryo Funayama,Masamitsu Maekawa,Hideaki Karasawa,Ryosuke Kashiwagi,Yayoi Aoyama,Nariyasu Mano,Shinobu Ohnuma,Michiaki Unno,Keiko Nakayama

Drug metabolism and disposition: the biological fate of chemicals 50:770-780 PubMed35307651

2022

Localization of Xenobiotic Transporters Expressed at the Human Blood-Testis Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Raymond K Hau,Robert R Klein,Stephen H Wright,Nathan J Cherrington

Nutrients 13: PubMed34069490

2021

Essential Oils, and , Chemosensitize Resistant Human Uterine Sarcoma MES-SA/Dx5 Cells to Doxorubicin by Inducing Apoptosis and Targeting P-Glycoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Lahmar,Aline Mathey,Virginie Aires,Dorra Elgueder,Anne Vejux,Rihab Khlifi,Fairouz Sioud,Leila Chekir-Ghedira,Dominique Delmas

Oncology letters 20:148 PubMed32934716

2020

Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Shi,Fang-Fang Xu,Cai-Ping Xiang,Ru Jia,Chun-Hong Yan,Se-Qing Ma,Ning Wang,An-Jiao Wang,Ping Fan

Oncology research 28:423-438 PubMed32331534

2020

The AKT/GSK3-Mediated Slug Expression Contributes to Oxaliplatin Resistance in Colorectal Cancer via Upregulation of ERCC1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Xiao-Dong Ma,Guan-Min Jiang,Bin Shi,Wen Zhong,Chun-Lei Sun,Liang Zhao,Yan-Jiao Hou,Hao Wang

Journal of cellular physiology 235:1374-1385 PubMed31506947

2019

MicroRNA-448 overexpression inhibits fibroblast proliferation and collagen synthesis and promotes cell apoptosis via targeting ABCC3 through the JNK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feihong Xu,Fei Xu,Shiguang Xie,Wei Zuo,Guilan Wen,Tiantian Zhao,Xuan Wan

The Journal of pharmacology and experimental therapeutics 370:269-277 PubMed31175220

2019

Mechanistic Basis of Cabotegravir-Glucuronide Disposition in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Mitesh Patel,H Christian Eberl,Andrea Wolf,Esaie Pierre,Joseph W Polli,Maciej J Zamek-Gliszczynski

Oncology letters 18:63-71 PubMed31289473

2019

Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Chun-Hua Chi,Ying Zhang,Bin Shi,Ru Jia,Ben-Jun Wang

Oncology letters 17:5572-5580 PubMed31186779

2019

Prognostic implication of ABC transporters and cancer stem cell markers in patients with stage III colon cancer receiving adjuvant FOLFOX-4 chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Song-Hee Han,Jin Won Kim,Milim Kim,Jee Hyun Kim,Keun-Wook Lee,Bo-Hyung Kim,Heung-Kwon Oh,Duck-Woo Kim,Sung-Bum Kang,Hyunchul Kim,Eun Shin

Cells 8: PubMed30884843

2019

Ursodeoxycholic Acid Regulates Hepatic Energy Homeostasis and White Adipose Tissue Macrophages Polarization in Leptin-Deficiency Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Sheng Chen,Hsuan-Miao Liu,Tzung-Yan Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MRP3 antibody [M3II-9]

Functional Studies - Anti-MRP3 antibody [M3II-9] (AB3375)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

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