Goat Polyclonal MRP4 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ABCC4.
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Publications
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- European journal of pharmacology 909:1743952021Antioxidant effect of ascorbic acid against cisplatin-induced nephrotoxicity and P-glycoprotein expression in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeisuke Okamoto,Fumi Kitaichi,Yoshitaka Saito,Hinata Ueda,Katsuya Narumi,Ayako Furugen,Masaki KobayashiPubMed 34332922
- Life sciences 232:1166432019High soluble endoglin levels regulate cholesterol homeostasis and bile acids turnover in the liver of transgenic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva Dolezelova et. al.PubMed 31299237
- Oncology letters 18:63-712019Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Wang et. al.PubMed 31289473
- Nature communications 8:156632017The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Castella et. al.PubMed 28580927
- British journal of pharmacology 173:497-5102016Blood-brain and retinal barriers show dissimilar ABC transporter impacts and concealed effect of P-glycoprotein on a novel verapamil influx carrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHélène Chapy,Bruno Saubaméa,Nicolas Tournier,Fanchon Bourasset,Francine Behar-Cohen,Xavier Declèves,Jean-Michel Scherrmann,Salvatore CisterninoPubMed 26507673
- PloS one 10:e01261592015An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells.
- Drug metabolism and disposition: the biological fate of chemicals 43:266-722014Renal xenobiotic transporter expression is altered in multiple experimental models of nonalcoholic steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark J Canet,Rhiannon N Hardwick,April D Lake,Anika L Dzierlenga,John D Clarke,Michael J Goedken,Nathan J CherringtonPubMed 25488932
- Blood 120:404-142012SLC35D3 delivery from megakaryocyte early endosomes is required for platelet dense granule biogenesis and is differentially defective in Hermansky-Pudlak syndrome models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRonghua Meng et. al.PubMed 22611153
- American journal of physiology. Gastrointestinal a 299:G126-352010Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ABCC4. The exact immunogen used to generate this antibody is proprietary information. Database link O15439
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4-6 µg/mL | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-3 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins (PubMed:11856762, PubMed:12523936, PubMed:12835412, PubMed:12883481, PubMed:15364914, PubMed:15454390, PubMed:16282361, PubMed:17959747, PubMed:18300232, PubMed:26721430). Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4 (PubMed:17959747). Mediates the cotransport of bile acids with reduced glutathione (GSH) (PubMed:12523936, PubMed:12883481, PubMed:16282361). Transports a wide range of drugs and their metabolites, including anticancer, antiviral and antibiotics molecules (PubMed:11856762, PubMed:12105214, PubMed:15454390, PubMed:17344354, PubMed:18300232). Confers resistance to anticancer agents such as methotrexate (PubMed:11106685).
Alternative names
MOATB, MRP4, ABCC4, ATP-binding cassette sub-family C member 4, MRP/cMOAT-related ABC transporter, Multi-specific organic anion transporter B, Multidrug resistance-associated protein 4, MOAT-B
Recommended products
Goat Polyclonal MRP4 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ABCC4.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ABCC4. The exact immunogen used to generate this antibody is proprietary information. Database link O15439
- Purification technique
- Affinity purification Immunogen
- Specificity
ab77184 is expected to recognize MRP4 isoform 1 (NP_005836.2) only.
- Concentration
- Purification notes
ab77184 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MRP4 protein also known as Multidrug Resistance Protein 4 or ABCC4 is an ATP-binding cassette transporter with a mass of approximately 150 kDa. This protein is expressed in various tissues including the liver kidney and brain. It plays a mechanical role by transporting organic anions drugs and other xenobiotic compounds across cellular membranes. MRP4 is part of the wider ATP-binding cassette (ABC) transporter family which actively pumps substrates against concentration gradients using ATP hydrolysis.
- Biological function summary
MRP4 impacts processes like pharmacokinetics and drug metabolism. The protein contributes to the efflux of endogenous substrates and medication from cells affecting their absorption and excretion. MRP4 also functions as part of a larger transporter complex working with other similar proteins to manage cellular detoxification systems. The MRP4 transporter maintains cellular homeostasis by removing harmful substances supporting cellular defense mechanisms against xenobiotic and endogenous compounds.
- Pathways
MRP4 integrates into cellular processes such as prostaglandin and cyclic nucleotide signaling pathways. These pathways are important in regulating inflammatory responses and cardiovascular functions. MRP4 interacts with other proteins including MRP1 and MRP5 which facilitate the movement of similar substrates providing flexibility and redundancy within cellular transport systems. This involvement enables MRP4 to modulate biological effects via alteration in prostaglandin levels.
- Associated diseases and disorders
MRP4 connects to conditions such as cancer and viral infections. Elevated expression of MRP4 can lead to drug resistance in cancer therapy complicating treatment strategies. In viral infections MRP4 modulates the availability of nucleoside analogs used in antiviral therapies. The protein's role aligns with other transporters like P-glycoprotein showcasing its involvement in the multidrug resistance phenotype. Consequently understanding MRP4's function can aid in the development of new therapeutic approaches targeting these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-MRP4 antibody (ab77184)
All lanes: Western blot - Anti-MRP4 antibody (ab77184) at 0.5 µg/mL
All lanes: rat renal cortex membranes at 20 µg
Predicted band size: 150 kDa
Observed band size: 170 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody (ab77184)
ab77184 (4µg/ml) staining of paraffin embedded Human Prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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