Anti-MRP4 antibody [EPR20403]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] (AB233382)

Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Basolateral membrane staining on human prostate epithelia (PMID : 18615486, PMID : 25840885). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] (AB233382)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling MRP4 with ab233382 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the A549 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] (AB233382)

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human ovarian cancer cells is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] (AB233382)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. MRP4 is expressed at the apical (brush border) membrane of human kidney tubules (PMID : 11856762). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] (AB233382)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling MRP4 with ab233382 at 1/50 dilution, followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the LNCaP cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• IP

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Immunoprecipitation - Anti-MRP4 antibody [EPR20403] (AB233382)

MRP4 was immunoprecipitated from 0.35 mg U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate with ab233382 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233382 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : U-2 OS whole cell lysate 10 μg (Input).

Lane 2 : ab233382 IP in U-2 OS whole cell lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab233382 in U-2 OS whole cell lysate (-).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

The molecular mass observed is consistent with the literature (PMID : 15827327).

All lanes:

Immunoprecipitation - Anti-MRP4 antibody [EPR20403] (ab233382)

Predicted band size: 150 kDa

false

• WB

Supplier Data

Western blot - Anti-MRP4 antibody [EPR20403] (AB233382)

Exposure time : Lanes 1-2 : 3 minutes, Lane 3 : 32 seconds, Lanes 4-5 : 2 minutes.

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with the literature (PMID : 15827327).

All lanes:

Western blot - Anti-MRP4 antibody [EPR20403] (ab233382) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

NIH:OVCAR-3 (human ovary adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 3:

A549 (human lung carcinoma cell line) whole cell lysate at 10 µg

Lane 4:

U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 20 µg

Lane 5:

LNCaP (human prostate cancer cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 150 kDa

Observed band size: 220-250 kDa

false