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Rabbit Recombinant Monoclonal MRP4 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples.

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Images

Immunoprecipitation - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (AB235624), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (AB235624), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (AB235624), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (AB235624), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (AB235624), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Expected
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins (PubMed:11856762, PubMed:12523936, PubMed:12835412, PubMed:12883481, PubMed:15364914, PubMed:15454390, PubMed:16282361, PubMed:17959747, PubMed:18300232, PubMed:26721430). Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4 (PubMed:17959747). Mediates the cotransport of bile acids with reduced glutathione (GSH) (PubMed:12523936, PubMed:12883481, PubMed:16282361). Transports a wide range of drugs and their metabolites, including anticancer, antiviral and antibiotics molecules (PubMed:11856762, PubMed:12105214, PubMed:15454390, PubMed:17344354, PubMed:18300232). Confers resistance to anticancer agents such as methotrexate (PubMed:11106685).

Alternative names

MOATB, MRP4, ABCC4, ATP-binding cassette sub-family C member 4, MRP/cMOAT-related ABC transporter, Multi-specific organic anion transporter B, Multidrug resistance-associated protein 4, MOAT-B

Recommended products

Rabbit Recombinant Monoclonal MRP4 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR20403
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab235624 is the carrier-free version of Anti-MRP4 antibody [EPR20403] ab233382.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The MRP4 protein also known as Multidrug Resistance Protein 4 or ABCC4 is an ATP-binding cassette transporter with a mass of approximately 150 kDa. This protein is expressed in various tissues including the liver kidney and brain. It plays a mechanical role by transporting organic anions drugs and other xenobiotic compounds across cellular membranes. MRP4 is part of the wider ATP-binding cassette (ABC) transporter family which actively pumps substrates against concentration gradients using ATP hydrolysis.

Biological function summary

MRP4 impacts processes like pharmacokinetics and drug metabolism. The protein contributes to the efflux of endogenous substrates and medication from cells affecting their absorption and excretion. MRP4 also functions as part of a larger transporter complex working with other similar proteins to manage cellular detoxification systems. The MRP4 transporter maintains cellular homeostasis by removing harmful substances supporting cellular defense mechanisms against xenobiotic and endogenous compounds.

Pathways

MRP4 integrates into cellular processes such as prostaglandin and cyclic nucleotide signaling pathways. These pathways are important in regulating inflammatory responses and cardiovascular functions. MRP4 interacts with other proteins including MRP1 and MRP5 which facilitate the movement of similar substrates providing flexibility and redundancy within cellular transport systems. This involvement enables MRP4 to modulate biological effects via alteration in prostaglandin levels.

Associated diseases and disorders

MRP4 connects to conditions such as cancer and viral infections. Elevated expression of MRP4 can lead to drug resistance in cancer therapy complicating treatment strategies. In viral infections MRP4 modulates the availability of nucleoside analogs used in antiviral therapies. The protein's role aligns with other transporters like P-glycoprotein showcasing its involvement in the multidrug resistance phenotype. Consequently understanding MRP4's function can aid in the development of new therapeutic approaches targeting these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624), expandable thumbnail

    Immunoprecipitation - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

    MRP4 was immunoprecipitated from 0.35 mg U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate with Anti-MRP4 antibody [EPR20403] ab233382 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-MRP4 antibody [EPR20403] ab233382 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: U-2 OS whole cell lysate 10 μg (Input).

    Lane 2: Anti-MRP4 antibody [EPR20403] ab233382 IP in U-2 OS whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MRP4 antibody [EPR20403] ab233382 in U-2 OS whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The molecular mass observed is consistent with the literature (PMID: 15827327).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MRP4 antibody [EPR20403] ab233382).

    All lanes: Immunoprecipitation - Anti-MRP4 antibody [EPR20403] (Anti-MRP4 antibody [EPR20403] ab233382)

    Predicted band size: 150 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling MRP4 with Anti-MRP4 antibody [EPR20403] ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human ovarian cancer cells is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MRP4 antibody [EPR20403] ab233382).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling MRP4 with Anti-MRP4 antibody [EPR20403] ab233382 at 1/50 dilution, followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the LNCaP cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MRP4 antibody [EPR20403] ab233382).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

    Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling MRP4 with Anti-MRP4 antibody [EPR20403] ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Basolateral membrane staining on human prostate epithelia (PMID:18615486, PMID:25840885). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MRP4 antibody [EPR20403] ab233382).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling MRP4 with Anti-MRP4 antibody [EPR20403] ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. MRP4 is expressed at the apical (brush border) membrane of human kidney tubules (PMID: 11856762). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MRP4 antibody [EPR20403] ab233382).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling MRP4 with Anti-MRP4 antibody [EPR20403] ab233382 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the A549 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MRP4 antibody [EPR20403] ab233382).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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