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AB230674

Anti-MRP5 antibody [6C6]

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(3 Publications)

Mouse Monoclonal MRP5 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ABCC5.

View Alternative Names

MRP5, ABCC5, ATP-binding cassette sub-family C member 5, Multi-specific organic anion transporter C, Multidrug resistance-associated protein 5, SMRP, pABC11, MOAT-C

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP5 antibody [6C6] (AB230674)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP5 antibody [6C6] (AB230674)

Formalin-fixed, paraffin-embedded human colon tissue stained for MRP5 protein using ab230674 at 10 μg/ml in immunohistochemical analysis.

Western blot - Anti-MRP5 antibody [6C6] (AB230674)
  • WB

Supplier Data

Western blot - Anti-MRP5 antibody [6C6] (AB230674)

All lanes:

Western blot - Anti-MRP5 antibody [6C6] (ab230674) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cell lysate at 5 µg

Lane 2:

HEK-293T transfected with pCMV6-ENTRY MRP5 cDNA cell lysate at 5 µg

Predicted band size: 161 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6C6

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ABCC5.

O15440

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRP5 also known as ABCC5 is part of the ATP-binding cassette (ABC) transporter family. This protein is approximately 162 kDa in mass and is involved in the transport of various molecules across cellular membranes. MRP5 is expressed widely in human tissues with significant expression seen in the brain lung and cardiovascular tissues. It acts as an organic anion transporter and has roles in cellular detoxification and the efflux of cyclic nucleotides and drugs which makes it relevant to pharmacology and chemotherapy resistance.
Biological function summary

MRP5 functions by transporting cyclic nucleotides such as cGMP and cAMP out of cells regulating their intracellular levels. It plays a role in modulating signal transduction processes and is also involved in the regulation of nitric oxide and the maintenance of vascular homeostasis. MRP5 does not form a complex with other proteins for its primary transport function operating largely as an independent transporter in cellular detoxification.

Pathways

MRP5 contributes significantly to the cAMP and cGMP signaling pathways where it influences vascular tone and cardiac function. It associates with proteins such as cGMP-dependent protein kinase through these pathways. Its activity in cyclic nucleotide transport impacts various signal transduction mechanisms that are essential for cell growth and communication.

MRP5 links to conditions like pulmonary hypertension and some forms of drug-resistant cancers. Its role in cyclic nucleotide efflux can affect vascular responses implicating it in pulmonary hypertension's pathogenesis. In the context of cancer increased MRP5 expression can lead to decreased effectiveness of chemotherapeutic agents. This resistance shares a connection with other ABC transporters involved in effluxing drugs out of cells such as P-glycoprotein. Understanding MRP5's role and regulation can offer insights into disease mechanisms and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds, and xenobiotics from cells. Mediates ATP-dependent transport of endogenous metabolites such as cAMP and cGMP, folic acid and N-lactoyl-amino acids (in vitro) (PubMed : 10893247, PubMed : 12637526, PubMed : 12695538, PubMed : 15899835, PubMed : 17229149, PubMed : 25964343). Also acts as a general glutamate conjugate and analog transporter that can limit the brain levels of endogenous metabolites, drugs, and toxins (PubMed : 26515061). Confers resistance to the antiviral agent PMEA (PubMed : 12695538). Able to transport several anticancer drugs including methotrexate, and nucleotide analogs in vitro, however it does with low affinity, thus the exact role of ABCC5 in mediating resistance still needs to be elucidated (PubMed : 10840050, PubMed : 12435799, PubMed : 12695538, PubMed : 15899835). Acts as a heme transporter required for the translocation of cytosolic heme to the secretory pathway (PubMed : 24836561). May play a role in energy metabolism by regulating the glucagon-like peptide 1 (GLP-1) secretion from enteroendocrine cells (By similarity).
See full target information ABCC5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:9688040 PubMed36193204

2022

Identification and Validation of Three-Gene Signature in Lung Squamous Cell Carcinoma by Integrated Transcriptome and Methylation Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghua Li,Libo Wu,Jiaxing Yu,Siyang Zhai,Hailong Deng,Qiushi Wang

Bioengineered 13:6567-6578 PubMed35227173

2022

HOTAIR mediates cisplatin resistance in nasopharyngeal carcinoma by regulating miR-106a-5p/SOX4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Cao,Yi Sun,Long Liu,Junwei Yu,Jiabiao Ji,Yatang Wang,Jianming Yang

Biology open : PubMed32229486

2020

Overexpression of long non-coding RNA SBF2-AS1 promotes cell progression in esophageal squamous cell carcinoma (ESCC) by repressing miR-494 to up-regulate PFN2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu Zhang,Xixiang Pan,Dongyang You
View all publications
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