AB24107

Anti-MRP5 antibody [M5II-54]

Name: Anti-MRP5 antibody [M5II-54] (ab24107) | Abcam
Brand: Abcam Limited
SKU: AB24107
Price: 500 USD
Availability: InStock

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(10 Publications)

Rat Monoclonal MRP5 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCC5 aa 700-950.

View Alternative Names

MRP5, ABCC5, ATP-binding cassette sub-family C member 5, Multi-specific organic anion transporter C, Multidrug resistance-associated protein 5, SMRP, pABC11, MOAT-C

1 Images

Flow Cytometry - Anti-MRP5 antibody [M5II-54] (AB24107)

Flow Cyt

Unknown

Flow Cytometry - Anti-MRP5 antibody [M5II-54] (AB24107)

Overlay histogram showing Y79 cells stained with ab24107 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24107, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H&L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Y79 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M5II-54

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human ABCC5 aa 700-950. The exact immunogen used to generate this antibody is proprietary information.

O15440

Epitope

ab24107 reacts with an internal epitope of MRP5. ab24107 does not cross-react with the human MDR1, MRP1, MRP2 or MRP3 gene products

Specificity

ab24107 does not cross-react with the human MDR1, MRP1, MRP2 or MRP3 gene products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

ab24107 has potential value for detection of MRP5-mediated drug-resistance in human tumor samples

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Antibody containing supernatant has been concentrated and filtered through a 0.22 micron filter and is serum free.

Storage buffer

pH: 7.3 Preservative: 0.1% Sodium azide Constituents: 0.7% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRP5 also known as ABCC5 is part of the ATP-binding cassette (ABC) transporter family. This protein is approximately 162 kDa in mass and is involved in the transport of various molecules across cellular membranes. MRP5 is expressed widely in human tissues with significant expression seen in the brain lung and cardiovascular tissues. It acts as an organic anion transporter and has roles in cellular detoxification and the efflux of cyclic nucleotides and drugs which makes it relevant to pharmacology and chemotherapy resistance.

Biological function summary

MRP5 functions by transporting cyclic nucleotides such as cGMP and cAMP out of cells regulating their intracellular levels. It plays a role in modulating signal transduction processes and is also involved in the regulation of nitric oxide and the maintenance of vascular homeostasis. MRP5 does not form a complex with other proteins for its primary transport function operating largely as an independent transporter in cellular detoxification.

Pathways

MRP5 contributes significantly to the cAMP and cGMP signaling pathways where it influences vascular tone and cardiac function. It associates with proteins such as cGMP-dependent protein kinase through these pathways. Its activity in cyclic nucleotide transport impacts various signal transduction mechanisms that are essential for cell growth and communication.

MRP5 links to conditions like pulmonary hypertension and some forms of drug-resistant cancers. Its role in cyclic nucleotide efflux can affect vascular responses implicating it in pulmonary hypertension's pathogenesis. In the context of cancer increased MRP5 expression can lead to decreased effectiveness of chemotherapeutic agents. This resistance shares a connection with other ABC transporters involved in effluxing drugs out of cells such as P-glycoprotein. Understanding MRP5's role and regulation can offer insights into disease mechanisms and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds, and xenobiotics from cells. Mediates ATP-dependent transport of endogenous metabolites such as cAMP and cGMP, folic acid and N-lactoyl-amino acids (in vitro) (PubMed : 10893247, PubMed : 12637526, PubMed : 12695538, PubMed : 15899835, PubMed : 17229149, PubMed : 25964343). Acts also as a general glutamate conjugate and analog transporter that can limit the brain levels of endogenous metabolites, drugs, and toxins (PubMed : 26515061). Confers resistance to the antiviral agent PMEA (PubMed : 12695538). Able to transport several anticancer drugs including methotrexate, and nucleotide analogs in vitro, however it does with low affinity, thus the exact role of ABCC5 in mediating resistance still needs to be elucidated (PubMed : 10840050, PubMed : 12435799, PubMed : 12695538, PubMed : 15899835). Acts as a heme transporter required for the translocation of cytosolic heme to the secretory pathway (PubMed : 24836561). May play a role in energy metabolism by regulating the glucagon-like peptide 1 (GLP-1) secretion from enteroendocrine cells (By similarity).

See full target information ABCC5

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37958530

2023

Delivery and Transcriptome Assessment of an In Vitro Three-Dimensional Proximal Tubule Model Established by Human Kidney 2 Cells in Clinical Gelatin Sponges.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Yi Hsiao,Tzung-Hai Yen,Fang-Yu Wu,Chao-Min Cheng,Jia-Wei Liu,Yu-Ting Fan,Jung-Ju Huang,Chung-Yi Nien

International journal of molecular sciences 24: PubMed37511019

2023

Use of Enzymatically Activated Carbon Monoxide Donors for Sensitizing Drug-Resistant Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Sodano,Barbara Rolando,Loretta Lazzarato,Costanzo Costamagna,Mariacristina Failla,Chiara Riganti,Konstantin Chegaev

Molecular therapy oncolytics 22:565-573 PubMed34553041

2021

Combined BRM270 and endostatin inhibit relapse of NSCLC while suppressing lung cancer stem cell proliferation induced by endostatin.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Hui Gu,Yong-Chun Shen,Yao Ou-Yang,Xi-Min Rao,Dan-Dan Fu,Fu-Qiang Wen

Oncology letters 20:167 PubMed32934734

2020

Emodin alleviates gemcitabine resistance in pancreatic cancer by inhibiting MDR1/P-glycoprotein and MRPs expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hongchun Guo,Feng Liu,Shuguang Yang,Tao Xue

EBioMedicine 44:403-418 PubMed31103629

2019

HNF1A inhibition induces the resistance of pancreatic cancer cells to gemcitabine by targeting ABCB1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Lu,Dongni Xu,Jintao Peng,Zhaofan Luo,Chujie Chen,Yuqing Chen,Huimou Chen,Minghui Zheng,Peihong Yin,Zhi Wang

OncoTargets and therapy 11:3853-3867 PubMed30013366

2018

Loss of miR-516a-3p mediates upregulation of ABCC5 in prostate cancer and drives its progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtuan Zhang,Zhenpeng Lian,Guangyu Sun,Ranlu Liu,Yong Xu

Toxicology 386:84-92 PubMed28587784

2017

Gender-specific expression of ATP-binding cassette (Abc) transporters and cytoprotective genes in mouse choroid plexus.

Applications

Unspecified application

Species

Unspecified reactive species

Katiria Flores,José E Manautou,J Larry Renfro

Oncology reports 35:2576-82 PubMed26985715

2016

Loss of Runt-related transcription factor 3 induces resistance to 5-fluorouracil and cisplatin in hepatocellular carcinoma.

Applications

IHC

Species

Unspecified reactive species

Junro Kataoka,Hidenori Shiraha,Shigeru Horiguchi,Hiroaki Sawahara,Daisuke Uchida,Teruya Nagahara,Masaya Iwamuro,Hiroki Morimoto,Yasuto Takeuchi,Kenji Kuwaki,Hideki Onishi,Shinichiro Nakamura,Akinobu Takaki,Kazuhiro Nouso,Takahito Yagi,Kazuhide Yamamoto,Hiroyuki Okada

The Journal of biological chemistry 289:28040-53 PubMed25138214

2014

Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Scott W Messenger,Michelle A Falkowski,Diana D H Thomas,Elaina K Jones,Wanjin Hong,Herbert Y Gaisano,Herbert Y Giasano,Nicholas M Boulis,Guy E Groblewski

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics 25:121-32 PubMed19323627

2009

Expression of multidrug resistance associated protein 5 (MRP5) on cornea and its role in drug efflux.

Applications

IP, IHC-P

Species

Human, Human

Pradeep K Karla,Tim L Quinn,Betty L Herndon,Priscilla Thomas,Dhananjay Pal,Ashim Mitra

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MRP5 antibody [M5II-54]

Flow Cytometry - Anti-MRP5 antibody [M5II-54] (AB24107)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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