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AB154961

Anti-MRPL12 antibody

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(3 Publications)

Rabbit Polyclonal MRPL12 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MRPL12 aa 1 to C-terminus.

View Alternative Names

MRPL7, RPML12, MRPL12, Large ribosomal subunit protein bL12m, 5c5-2, L12mt, MRP-L12

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL12 antibody (AB154961)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL12 antibody (AB154961)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling MRPL12 with ab154961 at 1/250.

Immunocytochemistry/ Immunofluorescence - Anti-MRPL12 antibody (AB154961)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRPL12 antibody (AB154961)

Immunofluorescence analysis of HeLa cells fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min labelling MRPL12 protein at mitochondria using ab154961 at a 1/500 dilution (Green).
Hoechst 33342 was used for nuclear staining (Blue).

Western blot - Anti-MRPL12 antibody (AB154961)
  • WB

Unknown

Western blot - Anti-MRPL12 antibody (AB154961)

12% SDS PAGE

All lanes:

Western blot - Anti-MRPL12 antibody (ab154961) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MRPL12 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P52815

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL12 also known as mitochondrial ribosomal protein L12 serves a mechanical function in the mitochondria. MRPL12 is part of the large 39S subunit of the mitochondrial ribosome. The protein has a mass of about 201 kDa and plays a role in protein synthesis by aiding the assembly of ribosomal RNA and proteins. MRPL12 is primarily expressed in tissues with high energy requirements such as the heart liver and skeletal muscle indicating its essential function in energy production processes.
Biological function summary

MRPL12 contributes to mitochondrial ribosome stability and the translation of mitochondrial-encoded proteins. MRPL12 forms interactions within the mitochondrial ribosome complex where it supports ribosomal assembly and function. It works closely with other mitochondrial ribosomal proteins to facilitate the efficient synthesis of proteins important for mitochondrial respiration and energy metabolism. This incorporation into the ribosome complex points to its significance in cellular energy production.

Pathways

MRPL12 is linked to mitochondrial protein synthesis and oxidative phosphorylation pathways. It partners with proteins such as MRPS12 within the ribosomal machinery to ensure the precise translation of mitochondrial mRNA. Its role is critical in maintaining the functional integrity of the electron transport chain which is necessary for ATP production. Proper functioning of these pathways supports cellular energy balance reinforcing the vital role of MRPL12 in cellular metabolism.

MRPL12 has connections to mitochondrial disorders and metabolic syndromes. Altered MRPL12 expression or mutations can impair mitochondrial protein synthesis leading to dysfunctional energy production and contributing to conditions such as mitochondrial myopathy and metabolic encephalopathy. MRPL12 interacts with proteins involved in these diseases such as MRPS12 suggesting a collective impact on mitochondrial function and highlighting the importance of accurate MRPL12 functioning in preventing energy-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As a component of the mitochondrial large ribosomal subunit, plays a role in mitochondrial translation (PubMed : 23603806). When present in mitochondria as a free protein not associated with the ribosome, associates with mitochondrial RNA polymerase POLRMT to activate transcription (PubMed : 22003127). Required for POLRMT stability (PubMed : 26586915).
See full target information MRPL12
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:5290 PubMed38906865

2024

RPLP1 restricts HIV-1 transcription by disrupting C/EBPβ binding to the LTR.

Applications

Unspecified application

Species

Unspecified reactive species

Weijing Yang,Hong Wang,Zhaolong Li,Lihua Zhang,Jianhui Liu,Frank Kirchhoff,Chen Huan,Wenyan Zhang

International journal of molecular sciences 24: PubMed36769082

2023

MRPL12 Acts as A Novel Prognostic Biomarker Involved in Immune Cell Infiltration and Tumor Progression of Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Hu,Yue Liu,Chenchao Ma,Kaixing Ai

Cell metabolism 33:1027-1041.e8 PubMed33770508

2021

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ping Wang,Azeem Sharda,Shuang-Nian Xu,Nick van Gastel,Cheuk Him Man,Una Choi,Wei Zhong Leong,Xi Li,David T Scadden
View all publications
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Product promise

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