JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB67844

Anti-MRPL18 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Mouse Polyclonal MRPL18 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MRPL18.

View Alternative Names

HSPC071, MRPL18, Large ribosomal subunit protein uL18m, L18mt, MRP-L18

2 Images
Western blot - Anti-MRPL18 antibody (AB67844)
  • WB

Unknown

Western blot - Anti-MRPL18 antibody (AB67844)

All lanes:

Western blot - Anti-MRPL18 antibody (ab67844) at 1/500 dilution

All lanes:

Human liver tissue lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

Predicted band size: 21 kDa

Observed band size: 18 kDa

true

Western blot - Anti-MRPL18 antibody (AB67844)
  • WB

Unknown

Western blot - Anti-MRPL18 antibody (AB67844)

All lanes:

Western blot - Anti-MRPL18 antibody (ab67844) at 1/500 dilution

Lane 1:

MRPL18 transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondaryantibody at 1/2500 dilution

Predicted band size: 21 kDa

Observed band size: 19 kDa,21 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MRPL18.

Q9H0U6

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL18 also known as mitochondrial ribosomal protein L18 is an integral component of the mitochondrial ribosome. This protein has a molecular mass of about 21 kDa. It plays a mechanical role in the translation of mitochondrial mRNA aiding the assembly of protein synthesis machinery. MRPL18 finds expression predominantly in tissues with high energy requirements such as heart and brain.
Biological function summary

MRPL18 is essential for mitochondrial protein biosynthesis. It forms part of the 39S subunit of the mitochondrial ribosome implicating its role in the production of polypeptides catalyzed by mitochondrial DNA. By facilitating efficient translation of genetic material into functional proteins MRPL18 collaborates with other ribosomal proteins and rRNA to maintain mitochondrial function.

Pathways

MRPL18 significantly contributes to oxidative phosphorylation and energy generation pathways. It supports these pathways by translating essential components of the electron transport chain. Closely related proteins like MRPL49 and MRPS16 also participate in these pathways together ensuring effective energy transduction within the cell.

MRPL18 shows associations with mitochondrial diseases such as mitochondrial encephalomyopathy. Impaired function or mutations in MRPL18 can disrupt mitochondrial protein synthesis leading to defective energy metabolism. The target's relationship with proteins like MRPS22 highlights its involvement in disease mechanisms affecting cellular energy homeostasis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Together with thiosulfate sulfurtransferase (TST), acts as a mitochondrial import factor for the cytosolic 5S rRNA. The precursor form shows RNA chaperone activity; is able to fold the 5S rRNA into an import-competent conformation that is recognized by rhodanese (TST). Both the cytoplasmic and mitochondrial forms are able to bind to the helix IV-loop D in the gamma domain of the 5S rRNA.
See full target information MRPL18
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science immunology 7:eabq4531 PubMed36399538

2022

mutations predispose to herpes simplex encephalitis by disrupting biogenesis of the host-derived RIG-I ligand .

Applications

Unspecified application

Species

Unspecified reactive species

Leslie Naesens,Santoshi Muppala,Dhiraj Acharya,Josephine Nemegeer,Delfien Bogaert,Jung-Hyun Lee,Katrien Staes,Veronique Debacker,Pieter De Bleser,Marieke De Bruyne,Elfride De Baere,Michiel van Gent,GuanQun Liu,Bart N Lambrecht,Jens Staal,Tessa Kerre,Rudi Beyaert,Jonathan Maelfait,Simon J Tavernier,Michaela U Gack,Filomeen Haerynck

Nature immunology 19:53-62 PubMed29180807

2017

Viral unmasking of cellular 5S rRNA pseudogene transcripts induces RIG-I-mediated immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica J Chiang,Konstantin M J Sparrer,Michiel van Gent,Charlotte Lässig,Teng Huang,Nikolaus Osterrieder,Karl-Peter Hopfner,Michaela U Gack

Genes & development 25:1289-305 PubMed21685364

2011

Biological significance of 5S rRNA import into human mitochondria: role of ribosomal protein MRP-L18.

Applications

WB

Species

Human

Alexandre Smirnov,Nina Entelis,Robert P Martin,Ivan Tarassov
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB247016

Anti-VPS36 antibody

Immunocytochemistry/ Immunofluorescence - Anti-VPS36 antibody (AB247016)

  • 0
  • 0
Primary Antibodies

AB176333

Anti-KDEL antibody [EPR12668]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [EPR12668] (AB176333)

  • 5
  • 3
Primary Antibodies

AB53288

Anti-GOSR1/GS28 antibody [EP1768Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (AB53288)

  • 4
  • 1
Primary Antibodies

AB118797

Anti-CEP128 antibody

Immunoprecipitation - Anti-CEP128 antibody (AB118797)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com