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AB250315

Anti-MRPL20 antibody [EPR12592] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MRPL20 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Large ribosomal subunit protein bL20m, L20mt, MRP-L20, MRPL20

1 Images
Western blot - Anti-MRPL20 antibody [EPR12592] - BSA and Azide free (AB250315)
  • WB

Supplier Data

Western blot - Anti-MRPL20 antibody [EPR12592] - BSA and Azide free (AB250315)

This data was developed using ab181058, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPL20 antibody [EPR12592] (<a href='/en-us/products/primary-antibodies/mrpl20-antibody-epr12592-ab181058'>ab181058</a>) at 1/1000 dilution

Lane 1:

HUVEC cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

RAW 264.7 cell lysate at 10 µg

Lane 5:

PC12 cell lysate at 10 µg

Lane 6:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12592

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250315 is the carrier-free version of ab181058.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL20 or mitochondrial ribosomal protein L20 is a component of the mitochondrial ribosome involved in protein synthesis within the mitochondria. MRPL20 weighs around 27 kDa and is key in the formation and function of the mitochondrial large ribosomal subunit. It is expressed ubiquitously with high levels in tissues that demand significant energy such as the brain heart and skeletal muscle.
Biological function summary

MRPL20 plays a role in protein assembly within mitochondria forming part of the 39S ribosomal subunit. It interacts with RNA and other ribosomal proteins to ensure proper mitochondrial protein translation. MRPL20 works in close relationship with other mitochondrial ribosomal proteins such as MRPL3 and MRPL15 facilitating efficient mitochondrial biogenesis and energy production.

Pathways

The MRPL20 protein is significant in the mitochondrial translation pathway which is important for respiratory chain complex assembly. It aids in encoding proteins necessary for oxidative phosphorylation. MRPL20 associates with proteins like MRPS12 and MRPS18A which participate in mitochondrial protein synthesis integrating into the broader mitochondrial function and energy production network.

MRPL20 is linked to mitochondrial dysfunction and related diseases such as mitochondrial encephalomyopathy and cardiomyopathy. These conditions can result from mutations affecting MRPL20 disrupting mitochondrial protein synthesis and impairing energy production. MRPL20 interacts with proteins like MFN2 which are also involved in mitochondrial maintenance and have roles in disorders related to energy deficits in the human body.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPL20
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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