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AB196842

Anti-MRPL28 antibody

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(2 Publications)

Rabbit Polyclonal MRPL28 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MRPL28.

View Alternative Names

MAAT1, MRPL28, Large ribosomal subunit protein bL28m, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes, L28mt, MRP-L28

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MRPL28 antibody (AB196842)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRPL28 antibody (AB196842)

Immunofluorescent analysis of HeLa cell labeling MRPL28 using ab196842 at a 1/50 dilution.

Blue : DAPI for nuclear staining.

Western blot - Anti-MRPL28 antibody (AB196842)
  • WB

Supplier Data

Western blot - Anti-MRPL28 antibody (AB196842)

All lanes:

Western blot - Anti-MRPL28 antibody (ab196842) at 1/500 dilution

Lane 1:

HeLa cell extract

Lane 2:

A375 cell extract

Lane 3:

mouse brain tissue extract

Lane 4:

mouse liver tissue extract

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MRPL28.

Q13084

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL28 also known as Mitochondrial Ribosomal Protein L28 is a component of the mitochondrial ribosome. It plays a critical role in protein synthesis within mitochondria. The protein has a molecular mass of about 32 kDa. MRPL28 is expressed in many tissues with higher expression levels observed in tissues with high energy demands such as the heart muscles and liver.
Biological function summary

MRPL28 integrates into the large subunit of the mitochondrial ribosome forming part of the machinery required for mitochondrial protein synthesis. This protein is essential for the production of proteins encoded by mitochondrial DNA. MRPL28 functions within the larger mitochondrial ribosomal complex which is distinct from cytoplasmic ribosomes highlighting its specialized role in maintaining mitochondrial function.

Pathways

The function of MRPL28 is closely tied to the oxidative phosphorylation pathway. This pathway involves the production of ATP via electron transport and chemiosmosis in the mitochondria. MRPL28 directly interacts with other mitochondrial ribosomal proteins to facilitate the translation of mitochondrial-encoded proteins essential for electron transport chain complexes. Changes in MRPL28 expression or function can impact energy metabolism and cellular homeostasis.

Mutations or dysregulation in MRPL28 have been associated with mitochondrial diseases such as mitochondrial myopathy. These disorders often affect energy-intensive tissues leading to symptoms such as muscle weakness or heart problems. Furthermore MRPL28 has connections to neurodegenerative diseases where mitochondrial dysfunction could play a role in disease progression. Understanding the link between MRPL28 and these conditions may offer insights into potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPL28
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Arthritis research & therapy 26:118 PubMed38851726

2024

Development of primary osteoarthritis during aging in genetically diverse UM-HET3 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sher Bahadur Poudel,Ryan R Ruff,Gozde Yildirim,Richard A Miller,David E Harrison,Randy Strong,Thorsten Kirsch,Shoshana Yakar

Cell death discovery 9:453 PubMed38086802

2023

A novel microtubule inhibitor promotes tumor ferroptosis by attenuating SLC7A11/GPX4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Ning,Ziqi Shang,Zhiping Liu,Zhizhou Xia,Yang Li,Ruibao Ren,Hongmei Wang,Yi Zhang
View all publications
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