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AB126719

Anti-MRPL28 antibody [EPR7578(B)]

5

(1 Review)

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(2 Publications)

Rabbit Recombinant Monoclonal MRPL28 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

MAAT1, MRPL28, Large ribosomal subunit protein bL28m, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes, L28mt, MRP-L28

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MRPL28 antibody [EPR7578(B)] (AB126719)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRPL28 antibody [EPR7578(B)] (AB126719)

ab126719, at 1/250 dilution, stains MRPL28 in A549 cells by immunofluorescence.

Western blot - Anti-MRPL28 antibody [EPR7578(B)] (AB126719)
  • WB

Unknown

Western blot - Anti-MRPL28 antibody [EPR7578(B)] (AB126719)

All lanes:

Western blot - Anti-MRPL28 antibody [EPR7578(B)] (ab126719) at 1/1000 dilution

Lane 1:

Fetal kidney tissue lysate

Lane 2:

JAR cell lysate

Lane 3:

HepG2 cell lysate

Lane 4:

A549 cell lysate

Lane 5:

293T (Human embryonic kidney epithelial cell) cell lysate

Predicted band size: 30 kDa

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OI-RD Scanning - Anti-MRPL28 antibody [EPR7578(B)] (AB126719)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MRPL28 antibody [EPR7578(B)] (AB126719)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7578(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL28 also known as Mitochondrial Ribosomal Protein L28 is a component of the mitochondrial ribosome. It plays a critical role in protein synthesis within mitochondria. The protein has a molecular mass of about 32 kDa. MRPL28 is expressed in many tissues with higher expression levels observed in tissues with high energy demands such as the heart muscles and liver.
Biological function summary

MRPL28 integrates into the large subunit of the mitochondrial ribosome forming part of the machinery required for mitochondrial protein synthesis. This protein is essential for the production of proteins encoded by mitochondrial DNA. MRPL28 functions within the larger mitochondrial ribosomal complex which is distinct from cytoplasmic ribosomes highlighting its specialized role in maintaining mitochondrial function.

Pathways

The function of MRPL28 is closely tied to the oxidative phosphorylation pathway. This pathway involves the production of ATP via electron transport and chemiosmosis in the mitochondria. MRPL28 directly interacts with other mitochondrial ribosomal proteins to facilitate the translation of mitochondrial-encoded proteins essential for electron transport chain complexes. Changes in MRPL28 expression or function can impact energy metabolism and cellular homeostasis.

Mutations or dysregulation in MRPL28 have been associated with mitochondrial diseases such as mitochondrial myopathy. These disorders often affect energy-intensive tissues leading to symptoms such as muscle weakness or heart problems. Furthermore MRPL28 has connections to neurodegenerative diseases where mitochondrial dysfunction could play a role in disease progression. Understanding the link between MRPL28 and these conditions may offer insights into potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPL28
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:867 PubMed36229446

2022

Anti-ANGPTL3-FLD monoclonal antibody treatment ameliorates podocyte lesions through attenuating mitochondrial damage.

Applications

Unspecified application

Species

Unspecified reactive species

Qianying Lv,Xinli Han,Jiajia Ni,Qianqian Ma,Rufeng Dai,Jiaojiao Liu,Jialu Liu,Yihui Zhai,Qian Shen,Li Sun,Haimei Liu,Jia Rao,Hong Xu

Cell reports 32:108154 PubMed32937139

2020

Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kim Schneider,Geoffrey Michael Nelson,Joseph Luke Watson,Jörg Morf,Maximillian Dalglish,Laura Martina Luh,Annika Weber,Anne Bertolotti
View all publications
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Product promise

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