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AB248145

Anti-MRPL28 antibody [EPR7578(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MRPL28 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MAAT1, MRPL28, Large ribosomal subunit protein bL28m, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes, L28mt, MRP-L28

3 Images
Western blot - Anti-MRPL28 antibody [EPR7578(B)] - BSA and Azide free (AB248145)
  • WB

Unknown

Western blot - Anti-MRPL28 antibody [EPR7578(B)] - BSA and Azide free (AB248145)

This data was developed using ab126719, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPL28 antibody [EPR7578(B)] (<a href='/en-us/products/primary-antibodies/mrpl28-antibody-epr7578b-ab126719'>ab126719</a>) at 1/1000 dilution

Lane 1:

Fetal kidney tissue lysate

Lane 2:

JAR cell lysate

Lane 3:

HepG2 cell lysate

Lane 4:

A549 cell lysate

Lane 5:

293T (Human embryonic kidney epithelial cell) cell lysate

Predicted band size: 30 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-MRPL28 antibody [EPR7578(B)] - BSA and Azide free (AB248145)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRPL28 antibody [EPR7578(B)] - BSA and Azide free (AB248145)

This data was developed using ab126719, the same antibody clone in a different buffer formulation.ab126719, at 1/250 dilution, stains MRPL28 in A549 cells by immunofluorescence.

OI-RD Scanning - Anti-MRPL28 antibody [EPR7578(B)] - BSA and Azide free (AB248145)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MRPL28 antibody [EPR7578(B)] - BSA and Azide free (AB248145)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7578(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab248145 is the carrier-free version of ab126719.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL28 also known as Mitochondrial Ribosomal Protein L28 is a component of the mitochondrial ribosome. It plays a critical role in protein synthesis within mitochondria. The protein has a molecular mass of about 32 kDa. MRPL28 is expressed in many tissues with higher expression levels observed in tissues with high energy demands such as the heart muscles and liver.
Biological function summary

MRPL28 integrates into the large subunit of the mitochondrial ribosome forming part of the machinery required for mitochondrial protein synthesis. This protein is essential for the production of proteins encoded by mitochondrial DNA. MRPL28 functions within the larger mitochondrial ribosomal complex which is distinct from cytoplasmic ribosomes highlighting its specialized role in maintaining mitochondrial function.

Pathways

The function of MRPL28 is closely tied to the oxidative phosphorylation pathway. This pathway involves the production of ATP via electron transport and chemiosmosis in the mitochondria. MRPL28 directly interacts with other mitochondrial ribosomal proteins to facilitate the translation of mitochondrial-encoded proteins essential for electron transport chain complexes. Changes in MRPL28 expression or function can impact energy metabolism and cellular homeostasis.

Mutations or dysregulation in MRPL28 have been associated with mitochondrial diseases such as mitochondrial myopathy. These disorders often affect energy-intensive tissues leading to symptoms such as muscle weakness or heart problems. Furthermore MRPL28 has connections to neurodegenerative diseases where mitochondrial dysfunction could play a role in disease progression. Understanding the link between MRPL28 and these conditions may offer insights into potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPL28
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:5945 PubMed36209205

2022

Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxu Ge,Jun Tan,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Yan Sun,Jing Luo,Chufeng Zhang,Liancai Zhu,Bochu Wang,Minxuan Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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