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AB151326

Anti-MRPL3 antibody

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(1 Publication)

Rabbit Polyclonal MRPL3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MRPL3 aa 150 to C-terminus.

View Alternative Names

MRL3, RPML3, MRPL3, Large ribosomal subunit protein uL3m, L3mt, MRP-L3

1 Images
Western blot - Anti-MRPL3 antibody (AB151326)
  • WB

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Western blot - Anti-MRPL3 antibody (AB151326)

10% SDS PAGE

All lanes:

Western blot - Anti-MRPL3 antibody (ab151326) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human MRPL3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P09001

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL3 also known as Mitochondrial Ribosomal Protein L3 functions mechanically as a component of the mitochondrial ribosome specifically the large 39S subunit. This protein plays a role in mitochondrial protein synthesis. MRPL3 has a molecular mass of approximately 35 kDa. It is expressed in the mitochondria across various tissues having a pivotal involvement in the synthesis of proteins essential for mitochondrial energy generation.
Biological function summary

MRPL3 is important for the production of mitochondrial proteins by facilitating the translation of mitochondrial mRNA molecules. It forms part of the mitochondrial ribosome complex which is distinct from cytoplasmic ribosomes due to its protein-rich composition and specific ribosomal RNA components. This unique ribosome composition allows MRPL3 to contribute to mitochondrial-specific translational mechanisms that support cellular respiration and energy production.

Pathways

MRPL3 is notably involved in mitochondrial translation and oxidative phosphorylation pathways. In the context of oxidative phosphorylation MRPL3 works alongside other mitochondrial ribosomal proteins like MRPS23 to ensure the proper translation of essential components of the electron transport chain. This pathway is important for ATP production which cells use as an energy source. Efficient functioning of these pathways relies on proteins produced through the process that involves MRPL3.

MRPL3 is associated with mitochondrial diseases and certain forms of cancer due to its role in energy metabolism and protein synthesis. Mutations or dysregulation in MRPL3 may lead to mitochondrial dysfunction resulting in conditions such as mitochondrial cardiomyopathy. In some cancers MRPL3 expression levels can alter linked with changes in cellular energy demands and protein synthesis. The protein MRPL44 has shown to interact with MRPL3 suggesting a contribution to the pathology when mitochondrial protein synthesis is impaired.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 293:17769-17779 PubMed30282801

2018

Sirtuin 7-mediated deacetylation of WD repeat domain 77 (WDR77) suppresses cancer cell growth by reducing WDR77/PRMT5 transmethylase complex activity.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Qi,Xiaoyan Shi,Miao Yu,Boya Liu,Minghui Liu,Shi Song,Shuaiyi Chen,Junhua Zou,Wei-Guo Zhu,Jianyuan Luo
View all publications
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