AB179819

Anti-MRPL30 antibody [EPR12502]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal MRPL30 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

MRPL28, RPML28, HSPC249, MRPL30, Large ribosomal subunit protein uL30m, L28mt, MRP-L28, L30mt, MRP-L30

1 Images

Supplier Data

Western blot - Anti-MRPL30 antibody [EPR12502] (AB179819)

All lanes:

Western blot - Anti-MRPL30 antibody [EPR12502] (ab179819) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 19 kDa

false

Carrier free

Anti-MRPL30 antibody [EPR12502] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12502

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.35% Sodium citrate, 0.17% Sodium chloride, 0.05% BSA, 0.03% EDTA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL30 also known as Mitochondrial Ribosomal Protein L30 is a component of the mitochondrial ribosome. This protein has a molecular mass of approximately 42.7 kDa and is encoded by the MRPL30 gene. Expression of MRPL30 occurs mainly in mitochondria across various tissues where it is involved in protein synthesis within the mitochondria. Its presence is important for the maintenance of mitochondrial function as it helps translate mitochondrial RNA into essential proteins.

Biological function summary

MRPL30 contributes to mitochondrial protein synthesis as a part of the large subunit in the mitochondrial ribosome complex. This complex differs from cytoplasmic ribosomes due to its unique protein-to-rRNA composition reflecting its specific role within mitochondria. MRPL30's involvement in the formation of the ribosomal subunit supports the production of proteins necessary for oxidative phosphorylation. This synthesis is essential for energy production and normal cellular metabolism.

Pathways

The functioning of MRPL30 integrates into mitochondrial protein synthesis and oxidative phosphorylation pathways. The protein ensures the efficient translation of mitochondrial-encoded genes important for ATP production through oxidative phosphorylation. It interacts with other ribosomal proteins such as MRPL28 and MRPL44 important for the assembly and function of the mitochondrial ribosome and thereby sustaining cellular energy demands.

Alterations in MRPL30 expression or function can link to mitochondrial dysfunction-related disorders including mitochondrial diseases. These diseases often result from impaired ATP production due to defective oxidative phosphorylation. Research also associates MRPL30 with neurodegenerative diseases as mitochondria play an important role in neuronal function. Its interaction with other mitochondrial ribosomal proteins like MRPS12 can affect mitochondrial translation stability contributing to the pathophysiology of such disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MRPL30

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell 186:3307-3324.e30 PubMed37385249

2023

Dynamic mapping of proteome trafficking within and between living cells by TransitID.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qin,Joleen S Cheah,Charles Xu,James Messing,Brian D Freibaum,Steven Boeynaems,J Paul Taylor,Namrata D Udeshi,Steven A Carr,Alice Y Ting

Cells 11: PubMed35805162

2022

Mitoribosomal Deregulation Drives Senescence via TPP1-Mediated Telomere Deprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Seongki Min,So Mee Kwon,Jiwon Hong,Young-Kyoung Lee,Tae Jun Park,Su Bin Lim,Gyesoon Yoon

Aging cell 20:e13306 PubMed33450132

2021

JUNB-FBXO21-ERK axis promotes cartilage degeneration in osteoarthritis by inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiming Lin,Jianing Miao,Tao Zhang,Ming He,Ziyuan Wang,Xinyuan Feng,Lunhao Bai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MRPL30 antibody [EPR12502]

Western blot - Anti-MRPL30 antibody [EPR12502] (AB179819)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise