JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250121

Anti-MRPL30 antibody [EPR12502] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal MRPL30 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MRPL28, RPML28, HSPC249, MRPL30, Large ribosomal subunit protein uL30m, L28mt, MRP-L28, L30mt, MRP-L30

1 Images
Western blot - Anti-MRPL30 antibody [EPR12502] - BSA and Azide free (AB250121)
  • WB

Supplier Data

Western blot - Anti-MRPL30 antibody [EPR12502] - BSA and Azide free (AB250121)

This data was developed using ab179819, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPL30 antibody [EPR12502] (<a href='/en-us/products/primary-antibodies/mrpl30-antibody-epr12502-ab179819'>ab179819</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12502

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

ab250121 is the carrier-free version of ab179819.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL30 also known as Mitochondrial Ribosomal Protein L30 is a component of the mitochondrial ribosome. This protein has a molecular mass of approximately 42.7 kDa and is encoded by the MRPL30 gene. Expression of MRPL30 occurs mainly in mitochondria across various tissues where it is involved in protein synthesis within the mitochondria. Its presence is important for the maintenance of mitochondrial function as it helps translate mitochondrial RNA into essential proteins.
Biological function summary

MRPL30 contributes to mitochondrial protein synthesis as a part of the large subunit in the mitochondrial ribosome complex. This complex differs from cytoplasmic ribosomes due to its unique protein-to-rRNA composition reflecting its specific role within mitochondria. MRPL30's involvement in the formation of the ribosomal subunit supports the production of proteins necessary for oxidative phosphorylation. This synthesis is essential for energy production and normal cellular metabolism.

Pathways

The functioning of MRPL30 integrates into mitochondrial protein synthesis and oxidative phosphorylation pathways. The protein ensures the efficient translation of mitochondrial-encoded genes important for ATP production through oxidative phosphorylation. It interacts with other ribosomal proteins such as MRPL28 and MRPL44 important for the assembly and function of the mitochondrial ribosome and thereby sustaining cellular energy demands.

Alterations in MRPL30 expression or function can link to mitochondrial dysfunction-related disorders including mitochondrial diseases. These diseases often result from impaired ATP production due to defective oxidative phosphorylation. Research also associates MRPL30 with neurodegenerative diseases as mitochondria play an important role in neuronal function. Its interaction with other mitochondrial ribosomal proteins like MRPS12 can affect mitochondrial translation stability contributing to the pathophysiology of such disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information MRPL30
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:12868-12884 PubMed32580945

2020

Adipose mesenchymal stem cell-derived extracellular vesicles containing microRNA-26a-5p target TLR4 and protect against diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yurui Duan,Qingyang Luo,Yun Wang,Yali Ma,Fang Chen,Xiaoguang Zhu,Jun Shi
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB315855

Anti-Aquaporin 5 antibody [RM1099]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [RM1099] (AB315855)

  • 0
  • 0
Primary Antibodies

AB248797

Anti-MRPS15 antibody [EPR9361] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS15 antibody [EPR9361] - BSA and Azide free (AB248797)

  • 0
  • 0
Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56
Primary Antibodies

AB137064

Anti-Ndufs4 antibody [EP7832]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EP7832] (AB137064)

  • 5
  • 14

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com