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AB250169

Anti-MRPL4 antibody [EPR13151] - BSA and Azide free

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Rabbit Recombinant Monoclonal MRPL4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

CDABP0091, CGI-28, MRPL4, Large ribosomal subunit protein uL4m, L4mt, MRP-L4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL4 antibody [EPR13151] - BSA and Azide free (AB250169)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL4 antibody [EPR13151] - BSA and Azide free (AB250169)

This data was developed using ab180165, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human thyroid tissue labeling MRPL4 using ab180165 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL4 antibody [EPR13151] - BSA and Azide free (AB250169)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL4 antibody [EPR13151] - BSA and Azide free (AB250169)

This data was developed using ab180165, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling MRPL4 using ab180165 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-MRPL4 antibody [EPR13151] - BSA and Azide free (AB250169)
  • WB

Supplier Data

Western blot - Anti-MRPL4 antibody [EPR13151] - BSA and Azide free (AB250169)

This data was developed using ab180165, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPL4 antibody [EPR13151] (<a href='/en-us/products/primary-antibodies/mrpl4-antibody-epr13151-ab180165'>ab180165</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HL60 cell lysate at 10 µg

Lane 4:

HT29 cell lysate at 10 µg

Predicted band size: 35 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13151

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab250169 is the carrier-free version of ab180165.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPL4 also known as 39S ribosomal protein L4 mitochondrial is a mitochondrial ribosomal protein. This protein is a component of the large 39S subunit of the mitochondrial ribosome and has a molecular mass of approximately 39 kDa. MRPL4 is primarily expressed in tissues with high energy demands including the heart and skeletal muscle. It plays a role in mitochondrial protein synthesis facilitating the translation of proteins encoded by mitochondrial DNA.
Biological function summary

MRPL4 assists in the assembly and stability of the mitochondrial ribosome forming part of the mitoribosome complex. This complex is essential for the synthesis of mitochondrial proteins which are critical for proper mitochondrial function. MRPL4 ensures accurate translation by binding to ribosomal RNA stabilizing the structure needed for efficient ribosomal activity. Its function is important for maintaining cellular energy production cellular growth and metabolism.

Pathways

MRPL4 participates significantly in the mitochondrial translation pathway. This pathway is integral to the oxidative phosphorylation process which is essential for ATP production in cells. MRPL4 interacts with other ribosomal proteins such as MRPL3 and MRPL12 facilitating the coordination of protein synthesis within the mitochondrion. This process influences cellular respiration and bioenergetics which are critical for adapting to energy requirements.

MRPL4 is associated with conditions like mitochondrial disorders and cardiac diseases. Alterations in MRPL4 expression or function can disrupt mitochondrial protein synthesis leading to impaired oxidative phosphorylation and energy production. Such mitochondrial dysfunction is linked to disorders like mitochondrial encephalomyopathy and cardiomyopathy. MRPL4's interactions with other ribosomal proteins ensure that changes in its structure or expression could influence disease progression making it a target for further research in mitochondrial medicine.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPL4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com