AB185942

Anti-MRPP3 antibody [EPR14321-30]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal MRPP3 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

KIAA0391, MRPP3, PRORP, Mitochondrial ribonuclease P catalytic subunit, Mitochondrial ribonuclease P protein 3, Protein only RNase P catalytic subunit, Mitochondrial RNase P protein 3

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] (AB185942)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] (AB185942)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling MRPP3 with ab185942 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

Supplier Data

Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] (AB185942)

Western blot analysis of MRPP3 in 293 cell lysate immunoprecipitated using ab185942 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of 293 lysate. Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] (ab185942)

Predicted band size: 67 kDa

false

Supplier Data

Western blot - Anti-MRPP3 antibody [EPR14321-30] (AB185942)

All lanes:

Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942) at 1/5000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 67 kDa

false

Supplier Data

Western blot - Anti-MRPP3 antibody [EPR14321-30] (AB185942)

All lanes:

Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942) at 1/1000 dilution

All lanes:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 67 kDa

false

Carrier free

Anti-MRPP3 antibody [EPR14321-30] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14321-30

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "Based on the sequence analysis by BLAST, ab185942 recognizes three isoforms with predicted MWs of 67KDa, 65KDa and 56KDa, respectively.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPP3 also known as mitochondrial RNase P protein 3 is part of the RNase P complex which processes precursor tRNA molecules by cleaving their 5' leader sequences. It is a single subunit with a molecular mass of about 58 kDa and is mainly found in mitochondria. The protein shows expression in various tissues indicating its essential role in mitochondrial function.

Biological function summary

MRPP3 plays a critical role in mitochondrial RNA processing acting together with MRPP1 and MRPP2 within the RNase P complex. This complex is responsible for the maturation of mitochondrial tRNAs which are necessary for mitochondrial protein synthesis. MRPP3 is important for the stabilization and function of the RNase P complex ensuring the accuracy of tRNA processing.

Pathways

Mitochondrial RNA processing involves the collaboration of MRPP3 with components such as MRPP1 and MRPP2. This processing is an important part of the mitochondrial translation pathway which governs the synthesis of proteins required for mitochondrial oxidative phosphorylation. MRPP3 intersects with other mitochondrial proteins involved in maintaining proper respiration and energy production.

Defects in MRPP3 function are associated with mitochondrial diseases notably those linked to deficiencies in mitochondrial processing. Mutations can impair mitochondrial tRNA processing leading to defects in protein synthesis and energy production implicated in conditions like mitochondrial myopathy. MRPP3's interaction with MRPP1 and MRPP2 is significant as alterations in these proteins can exacerbate mitochondrial disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic ribonuclease component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and PRORP/MRPP3, which cleaves tRNA molecules in their 5'-ends (PubMed : 18984158, PubMed : 25953853, PubMed : 34715011). The presence of TRMT10C/MRPP1, HSD17B10/MRPP2 is required to catalyze tRNA molecules in their 5'-ends (PubMed : 25953853).

See full target information PRORP

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6391 PubMed40640125

2025

Super-resolution microscopy of mitochondrial mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Stoldt,Frederike Maass,Michael Weber,Sven Dennerlein,Peter Ilgen,Jutta Gärtner,Aysenur Canfes,Sarah V Schweighofer,Daniel C Jans,Peter Rehling,Stefan Jakobs

Proceedings of the National Academy of Sciences of the United States of America 121:e2414187121 PubMed39503847

2024

Cytosolic dependent translation supports mitochondrial RNA processing.

Applications

Unspecified application

Species

Unspecified reactive species

Mads M Foged,Emeline Recazens,Sylvain Chollet,Miriam Lisci,George E Allen,Boris Zinshteyn,Doha Boutguetait,Christian Münch,Vamsi K Mootha,Alexis A Jourdain

Molecular cell 82:3646-3660.e9 PubMed36044900

2022

The human mitochondrial genome contains a second light strand promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Benedict G Tan,Christian D Mutti,Yonghong Shi,Xie Xie,Xuefeng Zhu,Pedro Silva-Pinheiro,Katja E Menger,Héctor Díaz-Maldonado,Wei Wei,Thomas J Nicholls,Patrick F Chinnery,Michal Minczuk,Maria Falkenberg,Claes M Gustafsson

Photobiomodulation, photomedicine, and laser surgery 40:543-553 PubMed35904935

2022

The 532 nm Laser Treatment Promotes the Proliferation of Tendon-Derived Stem Cells and Upregulates to Stimulate Tenogenic Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Li,Yiming Zhu,Qing Pei,Yuhao Deng,Tao Ni

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MRPP3 antibody [EPR14321-30]

Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] (AB185942)

Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] (AB185942)

Western blot - Anti-MRPP3 antibody [EPR14321-30] (AB185942)

Western blot - Anti-MRPP3 antibody [EPR14321-30] (AB185942)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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