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AB250850

Anti-MRPP3 antibody [EPR14321-30] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MRPP3 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0391, MRPP3, PRORP, Mitochondrial ribonuclease P catalytic subunit, Mitochondrial ribonuclease P protein 3, Protein only RNase P catalytic subunit, Mitochondrial RNase P protein 3

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] - BSA and Azide free (AB250850)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] - BSA and Azide free (AB250850)

This data was developed using ab185942, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of acetone-fixed HeLa cells labeling MRPP3 with ab185942 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] - BSA and Azide free (AB250850)
  • IP

Supplier Data

Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] - BSA and Azide free (AB250850)

This data was developed using ab185942, the same antibody clone in a different buffer formulation.

Western blot analysis of MRPP3 in 293 cell lysate immunoprecipitated using ab185942 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of 293 lysate. Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] (<a href='/en-us/products/primary-antibodies/mrpp3-antibody-epr14321-30-ab185942'>ab185942</a>)

Predicted band size: 67 kDa

false

Western blot - Anti-MRPP3 antibody [EPR14321-30] - BSA and Azide free (AB250850)
  • WB

Supplier Data

Western blot - Anti-MRPP3 antibody [EPR14321-30] - BSA and Azide free (AB250850)

This data was developed using ab185942, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPP3 antibody [EPR14321-30] (<a href='/en-us/products/primary-antibodies/mrpp3-antibody-epr14321-30-ab185942'>ab185942</a>) at 1/5000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 67 kDa

false

Western blot - Anti-MRPP3 antibody [EPR14321-30] - BSA and Azide free (AB250850)
  • WB

Supplier Data

Western blot - Anti-MRPP3 antibody [EPR14321-30] - BSA and Azide free (AB250850)

This data was developed using ab185942, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPP3 antibody [EPR14321-30] (<a href='/en-us/products/primary-antibodies/mrpp3-antibody-epr14321-30-ab185942'>ab185942</a>) at 1/1000 dilution

All lanes:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14321-30

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Based on the sequence analysis by BLAST, <a href='/en-us/products/primary-antibodies/mrpp3-antibody-epr14321-30-ab185942'>ab185942</a> recognizes three isoforms with predicted MWs of 67KDa, 65KDa and 56KDa, respectively.</p>" } } }
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Product details

ab250850 is the carrier-free version of ab185942.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPP3 also known as mitochondrial RNase P protein 3 is part of the RNase P complex which processes precursor tRNA molecules by cleaving their 5' leader sequences. It is a single subunit with a molecular mass of about 58 kDa and is mainly found in mitochondria. The protein shows expression in various tissues indicating its essential role in mitochondrial function.
Biological function summary

MRPP3 plays a critical role in mitochondrial RNA processing acting together with MRPP1 and MRPP2 within the RNase P complex. This complex is responsible for the maturation of mitochondrial tRNAs which are necessary for mitochondrial protein synthesis. MRPP3 is important for the stabilization and function of the RNase P complex ensuring the accuracy of tRNA processing.

Pathways

Mitochondrial RNA processing involves the collaboration of MRPP3 with components such as MRPP1 and MRPP2. This processing is an important part of the mitochondrial translation pathway which governs the synthesis of proteins required for mitochondrial oxidative phosphorylation. MRPP3 intersects with other mitochondrial proteins involved in maintaining proper respiration and energy production.

Defects in MRPP3 function are associated with mitochondrial diseases notably those linked to deficiencies in mitochondrial processing. Mutations can impair mitochondrial tRNA processing leading to defects in protein synthesis and energy production implicated in conditions like mitochondrial myopathy. MRPP3's interaction with MRPP1 and MRPP2 is significant as alterations in these proteins can exacerbate mitochondrial disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic ribonuclease component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and PRORP/MRPP3, which cleaves tRNA molecules in their 5'-ends (PubMed : 18984158, PubMed : 25953853, PubMed : 34715011). The presence of TRMT10C/MRPP1, HSD17B10/MRPP2 is required to catalyze tRNA molecules in their 5'-ends (PubMed : 25953853).
See full target information PRORP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com