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AB185941

Anti-MRPP3 antibody [EPR14321]

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(1 Publication)

Rabbit Recombinant Monoclonal MRPP3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0391, MRPP3, PRORP, Mitochondrial ribonuclease P catalytic subunit, Mitochondrial ribonuclease P protein 3, Protein only RNase P catalytic subunit, Mitochondrial RNase P protein 3

1 Images
Western blot - Anti-MRPP3 antibody [EPR14321] (AB185941)
  • WB

Supplier Data

Western blot - Anti-MRPP3 antibody [EPR14321] (AB185941)

All lanes:

Western blot - Anti-MRPP3 antibody [EPR14321] (ab185941) at 1/10000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

U87-MG cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

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  • Carrier free

    Anti-MRPP3 antibody [EPR14321] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14321

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPP3 also known as mitochondrial RNase P protein 3 is part of the RNase P complex which processes precursor tRNA molecules by cleaving their 5' leader sequences. It is a single subunit with a molecular mass of about 58 kDa and is mainly found in mitochondria. The protein shows expression in various tissues indicating its essential role in mitochondrial function.
Biological function summary

MRPP3 plays a critical role in mitochondrial RNA processing acting together with MRPP1 and MRPP2 within the RNase P complex. This complex is responsible for the maturation of mitochondrial tRNAs which are necessary for mitochondrial protein synthesis. MRPP3 is important for the stabilization and function of the RNase P complex ensuring the accuracy of tRNA processing.

Pathways

Mitochondrial RNA processing involves the collaboration of MRPP3 with components such as MRPP1 and MRPP2. This processing is an important part of the mitochondrial translation pathway which governs the synthesis of proteins required for mitochondrial oxidative phosphorylation. MRPP3 intersects with other mitochondrial proteins involved in maintaining proper respiration and energy production.

Defects in MRPP3 function are associated with mitochondrial diseases notably those linked to deficiencies in mitochondrial processing. Mutations can impair mitochondrial tRNA processing leading to defects in protein synthesis and energy production implicated in conditions like mitochondrial myopathy. MRPP3's interaction with MRPP1 and MRPP2 is significant as alterations in these proteins can exacerbate mitochondrial disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic ribonuclease component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and PRORP/MRPP3, which cleaves tRNA molecules in their 5'-ends (PubMed : 18984158, PubMed : 25953853, PubMed : 34715011). The presence of TRMT10C/MRPP1, HSD17B10/MRPP2 is required to catalyze tRNA molecules in their 5'-ends (PubMed : 25953853).
See full target information PRORP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Photobiomodulation, photomedicine, and laser surgery 40:543-553 PubMed35904935

2022

The 532 nm Laser Treatment Promotes the Proliferation of Tendon-Derived Stem Cells and Upregulates to Stimulate Tenogenic Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Li,Yiming Zhu,Qing Pei,Yuhao Deng,Tao Ni
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com