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AB242120

Anti-MRPS15 antibody

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(1 Publication)

Rabbit Polyclonal MRPS15 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MRPS15 aa 200 to C-terminus.

View Alternative Names

RPMS15, DC37, MRPS15, Small ribosomal subunit protein uS15m, MRP-S15, S15mt

2 Images
Immunoprecipitation - Anti-MRPS15 antibody (AB242120)
  • IP

Supplier Data

Immunoprecipitation - Anti-MRPS15 antibody (AB242120)

MRPS15 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab242120 used for IP at 6 μg per reaction. For WB 0.4 μg/ml.

Lane 1 : ab242120 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-MRPS15 antibody (ab242120)

Predicted band size: 30 kDa

false

Western blot - Anti-MRPS15 antibody (AB242120)
  • WB

Supplier Data

Western blot - Anti-MRPS15 antibody (AB242120)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-MRPS15 antibody (ab242120) at 0.04 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 30 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human MRPS15 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P82914

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to MRPS15 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPS15 also known as 28S ribosomal protein S15 is a component of the mitochondrial ribosome small subunit. It has a mass of approximately 16 kilodaltons. MRPS15 functions as a structural protein and plays a role in ribosome assembly and maintenance. Expression of MRPS15 is mainly located within mitochondria where it participates in mitochondrial protein synthesis.
Biological function summary

The MRPS15 protein resides within the small subunit of the mitochondrial ribosome contributing to the assembly of the ribosomal architecture essential for mitochondrial translation. MRPS15 as part of the mitoribosome facilitates the production of proteins encoded by mitochondrial DNA which are necessary for the oxidative phosphorylation pathway. Mitochondrial ribosomes differ from their prokaryotic counterparts by their higher protein-to-rRNA ratio and the absence of a 5S rRNA highlighting MRPS15’s role in this specialized organelle.

Pathways

MRPS15 is integral to the mitochondrial translation process directly affecting the oxidative phosphorylation pathway. This pathway produces ATP through the electron transport chain essential for cellular energy metabolism. MRPS15 associates with other mitochondrial ribosomal proteins in this pathway to ensure efficient mitochondrial protein synthesis and energy production. It exhibits functional associations with other mitoribosomal proteins like MRPL12 which regulate the translation of mitochondrial-encoded proteins.

Mutations and dysregulation of MRPS15 have been connected to mitochondrial diseases which are often characterized by impaired energy production. Diseases such as mitochondrial complex I deficiency arise from defects in mitochondrial translation involving MRPS15. Mutations can disrupt MRPS15’s role in protein synthesis within mitochondria leading to dysfunctional energy metabolism. These defects may involve interactions with other mitochondrial proteins like NDUFV1 linking them to associated disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPS15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 21:22 PubMed38989294

2024

Mitochondrial protein isoleucyl-tRNA synthetase 2 in tumor cells as a potential therapeutic target for cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojiao Meng,Bo Gao,Ning Li
View all publications
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