AB137070

Anti-MRPS15 antibody [EPR9361]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal MRPS15 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

RPMS15, DC37, MRPS15, Small ribosomal subunit protein uS15m, MRP-S15, S15mt

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS15 antibody [EPR9361] (AB137070)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS15 antibody [EPR9361] (AB137070)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling MRPS15 with ab137070 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MRPS15 antibody [EPR9361] (AB137070)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRPS15 antibody [EPR9361] (AB137070)

Immunofluorescent analysis of HeLa cells labelling MRPS15 with ab137070 at 1/250 dilution.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRPS15 antibody [EPR9361] (AB137070)

Immunofluorescent analysis of U-87 MG cells labelling MRPS15 with ab137070 at 1/250 dilution.

Unknown

Western blot - Anti-MRPS15 antibody [EPR9361] (AB137070)

All lanes:

Western blot - Anti-MRPS15 antibody [EPR9361] (ab137070) at 1/1000 dilution

Lane 1:

U-87 MG cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 30 kDa

true

Carrier free

Anti-MRPS15 antibody [EPR9361] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9361

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPS15 also known as 28S ribosomal protein S15 is a component of the mitochondrial ribosome small subunit. It has a mass of approximately 16 kilodaltons. MRPS15 functions as a structural protein and plays a role in ribosome assembly and maintenance. Expression of MRPS15 is mainly located within mitochondria where it participates in mitochondrial protein synthesis.

Biological function summary

The MRPS15 protein resides within the small subunit of the mitochondrial ribosome contributing to the assembly of the ribosomal architecture essential for mitochondrial translation. MRPS15 as part of the mitoribosome facilitates the production of proteins encoded by mitochondrial DNA which are necessary for the oxidative phosphorylation pathway. Mitochondrial ribosomes differ from their prokaryotic counterparts by their higher protein-to-rRNA ratio and the absence of a 5S rRNA highlighting MRPS15’s role in this specialized organelle.

Pathways

MRPS15 is integral to the mitochondrial translation process directly affecting the oxidative phosphorylation pathway. This pathway produces ATP through the electron transport chain essential for cellular energy metabolism. MRPS15 associates with other mitochondrial ribosomal proteins in this pathway to ensure efficient mitochondrial protein synthesis and energy production. It exhibits functional associations with other mitoribosomal proteins like MRPL12 which regulate the translation of mitochondrial-encoded proteins.

Mutations and dysregulation of MRPS15 have been connected to mitochondrial diseases which are often characterized by impaired energy production. Diseases such as mitochondrial complex I deficiency arise from defects in mitochondrial translation involving MRPS15. Mutations can disrupt MRPS15’s role in protein synthesis within mitochondria leading to dysfunctional energy metabolism. These defects may involve interactions with other mitochondrial proteins like NDUFV1 linking them to associated disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MRPS15

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38542224

2024

Mitochondrial Ribosomal Protein MRPS15 Is a Component of Cytosolic Ribosomes and Regulates Translation in Stressed Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Florian David,Emilie Roussel,Carine Froment,Tangra Draia-Nicolau,Françoise Pujol,Odile Burlet-Schiltz,Anthony K Henras,Eric Lacazette,Florent Morfoisse,Florence Tatin,Jean-Jacques Diaz,Frédéric Catez,Barbara Garmy-Susini,Anne-Catherine Prats

Cells 11: PubMed35805162

2022

Mitoribosomal Deregulation Drives Senescence via TPP1-Mediated Telomere Deprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Seongki Min,So Mee Kwon,Jiwon Hong,Young-Kyoung Lee,Tae Jun Park,Su Bin Lim,Gyesoon Yoon

Cell death & disease 12:1076 PubMed34772924

2021

MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness.

Applications

Species

Human

Seongki Min,Young-Kyoung Lee,Jiwon Hong,Tae Jun Park,Hyun Goo Woo,So Mee Kwon,Gyesoon Yoon

Cancer cell international 21:136 PubMed33632224

2021

Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jihui Chen,Zhipeng Wang,Shouhong Gao,Kejin Wu,Fang Bai,Qiqiang Zhang,Hongyu Wang,Qin Ye,Fengjing Xu,Hong Sun,Yunshu Lu,Yan Liu

The Journal of biological chemistry 292:20208-20217 PubMed28978646

2017

Lactate-mediated mitoribosomal defects impair mitochondrial oxidative phosphorylation and promote hepatoma cell invasiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Kyoung Lee,Jin J Lim,Un-Woo Jeoun,Seongki Min,Eun-Beom Lee,So Mee Kwon,Changhan Lee,Gyesoon Yoon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MRPS15 antibody [EPR9361]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS15 antibody [EPR9361] (AB137070)

Immunocytochemistry/ Immunofluorescence - Anti-MRPS15 antibody [EPR9361] (AB137070)

Immunocytochemistry/ Immunofluorescence - Anti-MRPS15 antibody [EPR9361] (AB137070)

Western blot - Anti-MRPS15 antibody [EPR9361] (AB137070)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise