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AB248797

Anti-MRPS15 antibody [EPR9361] - BSA and Azide free

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Rabbit Recombinant Monoclonal MRPS15 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

View Alternative Names

RPMS15, DC37, MRPS15, Small ribosomal subunit protein uS15m, MRP-S15, S15mt

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS15 antibody [EPR9361] - BSA and Azide free (AB248797)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS15 antibody [EPR9361] - BSA and Azide free (AB248797)

This data was developed using ab137070, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling MRPS15 with ab137070 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MRPS15 antibody [EPR9361] - BSA and Azide free (AB248797)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRPS15 antibody [EPR9361] - BSA and Azide free (AB248797)

This data was developed using ab137070, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling MRPS15 with ab137070 at 1/250 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MRPS15 antibody [EPR9361] - BSA and Azide free (AB248797)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRPS15 antibody [EPR9361] - BSA and Azide free (AB248797)

This data was developed using ab137070, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of U-87 MG cells labelling MRPS15 with ab137070 at 1/250 dilution.

Western blot - Anti-MRPS15 antibody [EPR9361] - BSA and Azide free (AB248797)
  • WB

Unknown

Western blot - Anti-MRPS15 antibody [EPR9361] - BSA and Azide free (AB248797)

This data was developed using ab137070, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPS15 antibody [EPR9361] (<a href='/en-us/products/primary-antibodies/mrps15-antibody-epr9361-ab137070'>ab137070</a>) at 1/1000 dilution

Lane 1:

U-87 MG cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 30 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9361

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248797 is the carrier-free version of ab137070.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPS15 also known as 28S ribosomal protein S15 is a component of the mitochondrial ribosome small subunit. It has a mass of approximately 16 kilodaltons. MRPS15 functions as a structural protein and plays a role in ribosome assembly and maintenance. Expression of MRPS15 is mainly located within mitochondria where it participates in mitochondrial protein synthesis.
Biological function summary

The MRPS15 protein resides within the small subunit of the mitochondrial ribosome contributing to the assembly of the ribosomal architecture essential for mitochondrial translation. MRPS15 as part of the mitoribosome facilitates the production of proteins encoded by mitochondrial DNA which are necessary for the oxidative phosphorylation pathway. Mitochondrial ribosomes differ from their prokaryotic counterparts by their higher protein-to-rRNA ratio and the absence of a 5S rRNA highlighting MRPS15’s role in this specialized organelle.

Pathways

MRPS15 is integral to the mitochondrial translation process directly affecting the oxidative phosphorylation pathway. This pathway produces ATP through the electron transport chain essential for cellular energy metabolism. MRPS15 associates with other mitochondrial ribosomal proteins in this pathway to ensure efficient mitochondrial protein synthesis and energy production. It exhibits functional associations with other mitoribosomal proteins like MRPL12 which regulate the translation of mitochondrial-encoded proteins.

Mutations and dysregulation of MRPS15 have been connected to mitochondrial diseases which are often characterized by impaired energy production. Diseases such as mitochondrial complex I deficiency arise from defects in mitochondrial translation involving MRPS15. Mutations can disrupt MRPS15’s role in protein synthesis within mitochondria leading to dysfunctional energy metabolism. These defects may involve interactions with other mitochondrial proteins like NDUFV1 linking them to associated disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPS15
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com