AB151693

Anti-MRPS16 antibody [EPR8835]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal MRPS16 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

RPMS16, CGI-132, MRPS16, Small ribosomal subunit protein bS16m, MRP-S16, S16mt

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-MRPS16 antibody [EPR8835] (AB151693)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRPS16 antibody [EPR8835] (AB151693)

Immunofluorescent analysis of HepG2 cells labeling MRPS16 with ab151693 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS16 antibody [EPR8835] (AB151693)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS16 antibody [EPR8835] (AB151693)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MRPS16 with ab151693 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-MRPS16 antibody [EPR8835] (AB151693)

All lanes:

Western blot - Anti-MRPS16 antibody [EPR8835] (ab151693) at 1/1000 dilution

Lane 1:

HT1080 cell lysate at 10 µg

Lane 2:

A673 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 15 kDa

false

Carrier free

Anti-MRPS16 antibody [EPR8835] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8835

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPS16 also known as mitochondrial ribosomal protein S16 is a component of the small subunit of the mitochondrial ribosome. This protein plays an important role in mitochondrial protein synthesis and exhibits a molecular mass of approximately 15 kDa. It is expressed in many tissues where energy production is essential such as the brain muscle and liver. It participates actively in the assembly of the mitochondrial ribosome facilitating the translation of ribosomal RNA into functional proteins important for mitochondrial function.

Biological function summary

Mitochondrial ribosomal protein S16 supports mitochondrial gene expression as part of the mitochondrial ribosomal small subunit. It works in concert with other mitochondrial ribosomal proteins to ensure the efficient translation of mitochondrial DNA-encoded proteins which are integral to oxidative phosphorylation. This protein functions as part of a larger complex the mammalian mitochondrial ribosome which maintains the cellular energy balance by promoting mitochondrial protein synthesis.

Pathways

MRPS16 is an important player in mitochondrial translation and energy production pathways. It contributes to the proper functioning of the oxidative phosphorylation pathway which is critical for ATP generation in cells. MRPS16 functionally interacts with other mitochondrial ribosomal proteins such as MRPS18 in these pathways enabling the full capability of the mitochondrial translation machinery to synthesize proteins necessary for electron transport chain assembly and maintenance.

Mitochondrial dysfunction involving MRPS16 can contribute to conditions such as mitochondrial encephalomyopathy and Leigh syndrome. These disorders often stem from impaired mitochondrial function and energy production where MRPS16 mutation may disrupt core cellular energy processes. The protein's relationship to these diseases highlights possible associations with related mitochondrial proteins like MRPL3 which may share overlapping roles or compensatory mechanisms in mitochondrial ribosome formation and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MRPS16

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastroenterology 29:3770-3792 PubMed37426316

2023

Mechanism of ELL-associated factor 2 and vasohibin 1 regulating invasion, migration, and angiogenesis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Liang Feng,Ming-Jun Sun,Bo-Yang Xu,Meng-Yuan Liu,Hui-Jing Zhang,Can Wu

Journal of Cancer 11:2032-2043 PubMed32127931

2020

MRPS16 facilitates tumor progression via the PI3K/AKT/Snail signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Wang,Junjun Li,Xiaobing Long,Liwu Jiao,Minghui Zhou,Kang Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MRPS16 antibody [EPR8835]

Immunocytochemistry/ Immunofluorescence - Anti-MRPS16 antibody [EPR8835] (AB151693)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS16 antibody [EPR8835] (AB151693)

Western blot - Anti-MRPS16 antibody [EPR8835] (AB151693)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise