JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB249017

Anti-MRPS16 antibody [EPR8835] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal MRPS16 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

View Alternative Names

RPMS16, CGI-132, MRPS16, Small ribosomal subunit protein bS16m, MRP-S16, S16mt

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MRPS16 antibody [EPR8835] - BSA and Azide free (AB249017)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRPS16 antibody [EPR8835] - BSA and Azide free (AB249017)

This data was developed using ab151693, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling MRPS16 with ab151693 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS16 antibody [EPR8835] - BSA and Azide free (AB249017)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS16 antibody [EPR8835] - BSA and Azide free (AB249017)

This data was developed using ab151693, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MRPS16 with ab151693 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-MRPS16 antibody [EPR8835] - BSA and Azide free (AB249017)
  • WB

Unknown

Western blot - Anti-MRPS16 antibody [EPR8835] - BSA and Azide free (AB249017)

This data was developed using ab151693, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPS16 antibody [EPR8835] (<a href='/en-us/products/primary-antibodies/mrps16-antibody-epr8835-ab151693'>ab151693</a>) at 1/1000 dilution

Lane 1:

HT1080 cell lysate at 10 µg

Lane 2:

A673 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8835

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB182160

Anti-MRPS35 antibody [EPR11731(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] (AB182160)

  • 0
  • 0
Primary Antibodies

AB191891

Anti-MRPS18B antibody [EPR19298]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (AB191891)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab249017 is the carrier-free version of ab151693.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPS16 also known as mitochondrial ribosomal protein S16 is a component of the small subunit of the mitochondrial ribosome. This protein plays an important role in mitochondrial protein synthesis and exhibits a molecular mass of approximately 15 kDa. It is expressed in many tissues where energy production is essential such as the brain muscle and liver. It participates actively in the assembly of the mitochondrial ribosome facilitating the translation of ribosomal RNA into functional proteins important for mitochondrial function.
Biological function summary

Mitochondrial ribosomal protein S16 supports mitochondrial gene expression as part of the mitochondrial ribosomal small subunit. It works in concert with other mitochondrial ribosomal proteins to ensure the efficient translation of mitochondrial DNA-encoded proteins which are integral to oxidative phosphorylation. This protein functions as part of a larger complex the mammalian mitochondrial ribosome which maintains the cellular energy balance by promoting mitochondrial protein synthesis.

Pathways

MRPS16 is an important player in mitochondrial translation and energy production pathways. It contributes to the proper functioning of the oxidative phosphorylation pathway which is critical for ATP generation in cells. MRPS16 functionally interacts with other mitochondrial ribosomal proteins such as MRPS18 in these pathways enabling the full capability of the mitochondrial translation machinery to synthesize proteins necessary for electron transport chain assembly and maintenance.

Mitochondrial dysfunction involving MRPS16 can contribute to conditions such as mitochondrial encephalomyopathy and Leigh syndrome. These disorders often stem from impaired mitochondrial function and energy production where MRPS16 mutation may disrupt core cellular energy processes. The protein's relationship to these diseases highlights possible associations with related mitochondrial proteins like MRPL3 which may share overlapping roles or compensatory mechanisms in mitochondrial ribosome formation and function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information MRPS16
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com