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AB249861

Anti-MRPS17 antibody [EPR12583] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MRPS17 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RPMS17, HSPC011, MRPS17, Small ribosomal subunit protein uS17m, MRP-S17, S17mt

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS17 antibody [EPR12583] - BSA and Azide free (AB249861)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS17 antibody [EPR12583] - BSA and Azide free (AB249861)

This data was developed using ab175207, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling MRPS17 with ab175207 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MRPS17 antibody [EPR12583] - BSA and Azide free (AB249861)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRPS17 antibody [EPR12583] - BSA and Azide free (AB249861)

This data was developed using ab175207, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of HeLa cells labeling MRPS17 with ab175207 at 1/100 dilution (green). DAPI nuclear staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS17 antibody [EPR12583] - BSA and Azide free (AB249861)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS17 antibody [EPR12583] - BSA and Azide free (AB249861)

This data was developed using ab175207, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling MRPS17 with ab175207 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-MRPS17 antibody [EPR12583] - BSA and Azide free (AB249861)
  • IP

Supplier Data

Immunoprecipitation - Anti-MRPS17 antibody [EPR12583] - BSA and Azide free (AB249861)

This data was developed using ab175207, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling MRPS17 with ab175207 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-MRPS17 antibody [EPR12583] (<a href='/en-us/products/primary-antibodies/mrps17-antibody-epr12583-ab175207'>ab175207</a>)

Predicted band size: 15 kDa

false

Western blot - Anti-MRPS17 antibody [EPR12583] - BSA and Azide free (AB249861)
  • WB

Supplier Data

Western blot - Anti-MRPS17 antibody [EPR12583] - BSA and Azide free (AB249861)

This data was developed using ab175207, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPS17 antibody [EPR12583] (<a href='/en-us/products/primary-antibodies/mrps17-antibody-epr12583-ab175207'>ab175207</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

U937 cell lysate at 10 µg

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12583

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab249861 is the carrier-free version of ab175207.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPS17 also known as mitochondrial ribosomal protein S17 is a component of the mitochondrial ribosome small subunit. It weighs approximately 17 kDa. MRPS17 expresses in tissues throughout the body especially in those with high-energy requirements like muscle and brain. MRPS17 is essential for mitochondrial protein synthesis where it contributes to translating mitochondrial-encoded messages into functional proteins.
Biological function summary

The protein MRPS17 acts as part of the mitochondrial ribosome which contains many different proteins and ribosomal RNA. This complex plays a significant role in cellular energy metabolism by producing proteins essential for the electron transport chain. MRPS17 ensures that mitochondria maintain their capacity to synthesize proteins that drive ATP production. Without this process cells would lack adequate energy supply.

Pathways

MRPS17 engages in critical roles within mitochondrial translation pathway and oxidative phosphorylation pathway. These pathways facilitate the proper function of mitochondria in energy production. MRPS17 interacts with other mitochondrial ribosomal proteins like MRPS18 ensuring the accuracy and efficiency of protein synthesis. This interaction supports overall mitochondrial and cellular health.

Defects in MRPS17 relate to mitochondrial diseases such as Leigh syndrome and mitochondrial cardiomyopathy. These conditions often arise from disrupted energy production in cells. Genetic mutations affecting MRPS17 might impair mitochondrial function leading to clinical symptoms. MRPS17 shares connections with other mitochondrial proteins like MRPS16 in the context of these disorders highlighting its relevance in related pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPS17
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com