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AB191891

Anti-MRPS18B antibody [EPR19298]

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(1 Publication)

Rabbit Recombinant Monoclonal MRPS18B antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

C6orf14, HSPC183, PTD017, MRPS18B, Small ribosomal subunit protein mS40, Small ribosomal subunit protein bS18b, MRP-S18-2, MRP-S18-b, Mrps18-b, S18mt-b

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (AB191891)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (AB191891)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling MRPS18B with ab191891 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on HeLa cell line.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
1. ab191891 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
2. ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (AB191891)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (AB191891)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cell line labeling MRPS18B with ab191891 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on MCF7 cell line.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
1. ab191891 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
2. ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Immunoprecipitation - Anti-MRPS18B antibody [EPR19298] (AB191891)
  • IP

Supplier Data

Immunoprecipitation - Anti-MRPS18B antibody [EPR19298] (AB191891)

MRPS18B was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab191891 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab191891 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : MCF7 whole cell lysate, 10ug (Input).
Lane 2 : ab191891 IP in MCF7 whole cell lysate.
Lane 3 : Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab191891 in MCF7 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-MRPS18B antibody [EPR19298] (ab191891)

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891)
  • WB

Supplier Data

Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891)

5% NFDM/TBST : Blocking and dilution buffer.

All lanes:

Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 3min

Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891)
  • WB

Supplier Data

Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891)

5% NFDM/TBST : Blocking and dilution buffer.

All lanes:

Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 3min

Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891)
  • WB

Supplier Data

Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891)

5% NFDM/TBST : Blocking and dilution buffer.

All lanes:

Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Lane 3:

Mouse spleen tissue lysate at 10 µg

Lane 4:

Rat kidney tissue lysate at 10 µg

Lane 5:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 3min

Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891)
  • WB

Supplier Data

Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891)

5% NFDM/TBST : Blocking and dilution buffer.

All lanes:

Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 15s

  • Carrier free

    Anti-MRPS18B antibody [EPR19298] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19298

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB249017

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Immunocytochemistry/ Immunofluorescence - Anti-MRPS16 antibody [EPR8835] - BSA and Azide free (AB249017)

  • 0
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Primary Antibodies

AB182160

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] (AB182160)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB195810

Anti-TNFAIP8 antibody [EPR10058(3)]

RabMAb|Recombinant|Trial Size

Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MRPS18B protein also known as mitochondrial ribosomal protein S18B is a component of the mitochondrial ribosome with a molecular mass of approximately 27 kDa. This protein is an integral part of the small subunit of the mitochondrial ribosome (28S) which plays a role in protein synthesis within mitochondria. MRPS18B is expressed mainly in tissues with high energy demands such as the heart and skeletal muscle given its function in energy production.
Biological function summary

Proteins like MRPS18B are essential in maintaining mitochondrial function. MRPS18B operates within the ribosomal complex enabling the translation of mitochondrial-encoded genes necessary for proper cellular respiration. This role is critical for ATP production which supports numerous cellular processes. It shares functional similarities with other mitochondrial ribosomal proteins and together they ensure the synthesis of key components of the oxidative phosphorylation machinery.

Pathways

MRPS18B plays a significant role in the mitochondrial translation pathway. This pathway is important for the production of proteins encoded by the mitochondrial genome which are vital for the oxidative phosphorylation pathway. MRPS18B collaborates closely with other mitochondrial ribosomal proteins to facilitate accurate mRNA translation and is indirectly linked to the expression of proteins in the electron transport chain such as cytochrome c oxidase.

Abnormalities in MRPS18B expression or function can impact mitochondrial efficiency and have been connected to mitochondrial diseases such as Leigh syndrome. This syndrome is a neurodegenerative disorder that severely affects energy production in cells. MRPS18B also shows a relationship with Parkinson's disease where mitochondrial dysfunction plays a pivotal role with interactions noted between MRPS18B and proteins involved in mitochondrial maintenance such as PINK1 and Parkin.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPS18B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7929 PubMed36566259

2022

A genome-wide CRISPR screen identifies WDFY3 as a regulator of macrophage efferocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianting Shi,Xun Wu,Ziyi Wang,Fang Li,Yujiao Meng,Rebecca M Moore,Jian Cui,Chenyi Xue,Katherine R Croce,Arif Yurdagul,John G Doench,Wei Li,Konstantinos S Zarbalis,Ira Tabas,Ai Yamamoto,Hanrui Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com