Skip to main content

Rabbit Recombinant Monoclonal MRPS18B antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891), expandable thumbnail
  • Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891), expandable thumbnail
  • Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891), expandable thumbnail
  • Western blot - Anti-MRPS18B antibody [EPR19298] (AB191891), expandable thumbnail
  • Immunoprecipitation - Anti-MRPS18B antibody [EPR19298] (AB191891), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/40
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Rat
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Alternative names

C6orf14, HSPC183, PTD017, MRPS18B, Small ribosomal subunit protein mS40, Small ribosomal subunit protein bS18b, MRP-S18-2, MRP-S18-b, Mrps18-b, S18mt-b

Recommended products

Rabbit Recombinant Monoclonal MRPS18B antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19298
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The MRPS18B protein also known as mitochondrial ribosomal protein S18B is a component of the mitochondrial ribosome with a molecular mass of approximately 27 kDa. This protein is an integral part of the small subunit of the mitochondrial ribosome (28S) which plays a role in protein synthesis within mitochondria. MRPS18B is expressed mainly in tissues with high energy demands such as the heart and skeletal muscle given its function in energy production.

Biological function summary

Proteins like MRPS18B are essential in maintaining mitochondrial function. MRPS18B operates within the ribosomal complex enabling the translation of mitochondrial-encoded genes necessary for proper cellular respiration. This role is critical for ATP production which supports numerous cellular processes. It shares functional similarities with other mitochondrial ribosomal proteins and together they ensure the synthesis of key components of the oxidative phosphorylation machinery.

Pathways

MRPS18B plays a significant role in the mitochondrial translation pathway. This pathway is important for the production of proteins encoded by the mitochondrial genome which are vital for the oxidative phosphorylation pathway. MRPS18B collaborates closely with other mitochondrial ribosomal proteins to facilitate accurate mRNA translation and is indirectly linked to the expression of proteins in the electron transport chain such as cytochrome c oxidase.

Associated diseases and disorders

Abnormalities in MRPS18B expression or function can impact mitochondrial efficiency and have been connected to mitochondrial diseases such as Leigh syndrome. This syndrome is a neurodegenerative disorder that severely affects energy production in cells. MRPS18B also shows a relationship with Parkinson's disease where mitochondrial dysfunction plays a pivotal role with interactions noted between MRPS18B and proteins involved in mitochondrial maintenance such as PINK1 and Parkin.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891), expandable thumbnail

    Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891)

    5% NFDM/TBST: Blocking and dilution buffer.

    All lanes: Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

    Exposure time: 3min

  • Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891), expandable thumbnail

    Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891)

    5% NFDM/TBST: Blocking and dilution buffer.

    All lanes: Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

    Exposure time: 3min

  • Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891), expandable thumbnail

    Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891)

    5% NFDM/TBST: Blocking and dilution buffer.

    All lanes: Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

    Lane 1: Mouse heart tissue lysate at 10 µg

    Lane 2: Mouse kidney tissue lysate at 10 µg

    Lane 3: Mouse spleen tissue lysate at 10 µg

    Lane 4: Rat kidney tissue lysate at 10 µg

    Lane 5: Rat spleen tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

    Exposure time: 3min

  • Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891), expandable thumbnail

    Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891)

    5% NFDM/TBST: Blocking and dilution buffer.

    All lanes: Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

    Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 2: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-MRPS18B antibody [EPR19298] (ab191891), expandable thumbnail

    Immunoprecipitation - Anti-MRPS18B antibody [EPR19298] (ab191891)

    MRPS18B was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab191891 at 1/40 dilution.
    Western blot was performed from the immunoprecipitate using ab191891 at 1/1000 dilution.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
    Lane 1: MCF7 whole cell lysate, 10ug (Input).
    Lane 2: ab191891 IP in MCF7 whole cell lysate.
    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab191891 in MCF7 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-MRPS18B antibody [EPR19298] (ab191891)

    Predicted band size: 29 kDa

    Observed band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (ab191891), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (ab191891)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling MRPS18B with ab191891 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    1. ab191891 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
    2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (ab191891), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (ab191891)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cell line labeling MRPS18B with ab191891 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on MCF7 cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    1. ab191891 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
    2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com