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AB153940

Anti-MRPS27 antibody

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(2 Publications)

Rabbit Polyclonal MRPS27 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MRPS27 aa 1-200.

View Alternative Names

KIAA0264, MRPS27, Small ribosomal subunit protein mS27, Mitochondrial ribosomal protein S27, MRP-S27, S27mt

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS27 antibody (AB153940)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS27 antibody (AB153940)

Immunohistochemistry analysis of paraffin-embedded Human Colon carcinoma tissue labeling MRPS27 with ab153940 at 1/250 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MRPS27 antibody (AB153940)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MRPS27 antibody (AB153940)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling MRPS27 with ab153940 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Western blot - Anti-MRPS27 antibody (AB153940)
  • WB

Unknown

Western blot - Anti-MRPS27 antibody (AB153940)

12% SDS PAGE

All lanes:

Western blot - Anti-MRPS27 antibody (ab153940) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MRPS27 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q92552

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR7839] (AB129153)

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Primary Antibodies

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Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

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Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)

  • 5
  • 12
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPS27 also known as Mitochondrial Ribosomal Protein S27 is a protein that plays an essential role in the mitochondrial translation machinery. It is a component of the small mitochondrial ribosomal subunit. MRPS27 has a molecular mass of approximately 23 kDa. This protein is encoded by nuclear DNA and expressed in various tissues with higher levels in tissues having high energy demands like muscle and brain tissues.
Biological function summary

MRPS27 contributes to the formation and function of the mitochondrial ribosome a complex responsible for synthesizing proteins encoded by mitochondrial DNA. It is an integral part of the mitochondrial ribosomal small subunit where it participates in the initiation and elongation phases of mitochondrial protein synthesis. This indicates its importance in maintaining mitochondrial function and cellular energy metabolism.

Pathways

MRPS27 plays a role in the mitochondrial translation pathway where it contributes to the proper synthesis of proteins necessary for the electron transport chain. It interacts with other ribosomal proteins such as MRPS18 and MRPS9 to ensure effective translation processes within mitochondria. These pathways are critical for producing ATP through oxidative phosphorylation a central biochemical process in metabolism.

Defects in MRPS27 have associations with mitochondrial-related diseases including mitochondrial myopathy and encephalopathy. These conditions often arise due to impaired protein synthesis within the mitochondria affecting energy production. Additionally MRPS27 interacts with other proteins like MFN2 which is involved in maintaining mitochondrial network stability and mutations in MFN2 can lead to similar neuromuscular disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis (PubMed : 22841715). Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain (PubMed : 22841715). Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu) (PubMed : 22841715). Involved also in positive regulation of cell proliferation and tumor cell growth (PubMed : 28714366).
See full target information MRPS27
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 133: PubMed37581935

2023

CFTR-rich ionocytes mediate chloride absorption across airway epithelia.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Lei,Soumba Traore,Guillermo S Romano Ibarra,Philip H Karp,Tayyab Rehman,David K Meyerholz,Joseph Zabner,David A Stoltz,Patrick L Sinn,Michael J Welsh,Paul B McCray,Ian M Thornell

Viruses 15: PubMed37515107

2023

Kunjin Virus, Zika Virus, and Yellow Fever Virus Infections Have Distinct Effects on the Coding Transcriptome and Proteome of Brain-Derived U87 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin Brand,Gabrielle Deschamps-Francoeur,Kristen M Bullard-Feibelman,Michelle S Scott,Brian J Geiss,Martin Bisaillon
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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