JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB249421

Anti-MRPS31 antibody [EPR10707] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal MRPS31 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

IMOGN38, MRPS31, Small ribosomal subunit protein mS31, Imogen 38, MRP-S31, S31mt

1 Images
Western blot - Anti-MRPS31 antibody [EPR10707] - BSA and Azide free (AB249421)
  • WB

Unknown

Western blot - Anti-MRPS31 antibody [EPR10707] - BSA and Azide free (AB249421)

This data was developed using ab167406, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPS31 antibody [EPR10707] (<a href='/en-us/products/primary-antibodies/mrps31-antibody-epr10707-ab167406'>ab167406</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10707

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Product details

ab249421 is the carrier-free version of ab167406.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPS31 or mitochondrial ribosomal protein S31 is a component of the mitochondrial ribosome small subunit. Its molecular mass is approximately 45 kDa. MRPS31 is important in the synthesis of mitochondrial proteins since it facilitates protein translation within mitochondria. It mainly expresses in tissues with high energy demands such as muscle and brain. Mitochondrial ribosomal protein S31 is essential for maintaining mitochondrial function under different energy requirements.
Biological function summary

MRPS31 participates in the assembly and function of the mitochondrial ribosome a complex responsible for protein synthesis in mitochondria. It interacts closely with other ribosomal proteins and rRNA to ensure proper mitochondrial translation. By engaging in mitochondrial ribosome assembly MRPS31 contributes significantly to energy production and cellular metabolism supporting the high-energy needs of various types of cells.

Pathways

MRPS31 plays a significant role within oxidative phosphorylation and mitochondrial protein synthesis pathways. Oxidative phosphorylation relies on the proteins produced by the mitochondrial ribosome with MRPS31 aiding their synthesis. The mitochondrial ribosome including MRPS31 collaborates with proteins such as COX1 in the respiratory chain to ensure efficient energy generation. This integration into critical pathways emphasizes its role in cellular energy regulation and homeostasis.

MRPS31 is implicated in mitochondrial dysfunctions and neurodegenerative diseases. Mutations or dysfunctions in MRPS31 sometimes lead to disorders like mitochondrial myopathy where energy production becomes impaired. Moreover the protein's association with energy metabolism links it to Parkinson’s disease where mitochondrial deficits play a role. Direct interactions with proteins such as PINK1 in such conditions hint at its participation in disease pathogenesis and highlight its relevance in maintaining mitochondrial health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information MRPS31
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com