AB182160

Anti-MRPS35 antibody [EPR11731(2)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal MRPS35 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

MRPS28, HDCMD11P, MDS023, PSEC0213, MRPS35, Small ribosomal subunit protein mS35, MRP-S28, S28mt, MRP-S35, S35mt

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] (AB182160)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] (AB182160)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MRPS35 with ab182160 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] (AB182160)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling MRPS35 with ab182160 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MRPS35 antibody [EPR11731(2)] (AB182160)

All lanes:

Western blot - Anti-MRPS35 antibody [EPR11731(2)] (ab182160) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Carrier free

Anti-MRPS35 antibody [EPR11731(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11731(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPS35 also known as 28S ribosomal protein S35 or mitochondrial ribosomal protein S35 is a component of the small subunit of the mitochondrial ribosome. This protein is integral to mitochondrial protein synthesis and weighs approximately 39 kDa. It is expressed widely in human tissues but shows higher expression in tissues with high energy demand like skeletal muscle and heart. MRPS35 plays a mechanical role in facilitating the translation of mitochondrial mRNA to proteins necessary for mitochondrial function.

Biological function summary

MRPS35 is essential in mitochondrial ribosome assembly and protein synthesis. As part of the mitochondrial ribosomal small subunit it contributes to the assembly of ribosomes and the translation of essential proteins for mitochondrial energy production. This protein partners with other mitochondrial ribosomal proteins and plays a role in the synthesis of oxidative phosphorylation components an important metabolic pathway for ATP production.

Pathways

MRPS35 is significant in cellular respiratory processes. It plays a role in the oxidative phosphorylation pathway which is important for ATP production. MRPS35 interacts with other mitochondrial ribosomal proteins like MRPS30 to ensure efficient assembly and function of the mitochondrial ribosome. The proper synthesis of mitochondrial proteins is critical for maintaining the electron transport chain's function.

MRPS35 relates to certain mitochondrial disorders and possibly metabolic syndromes. Defects in mitochondrial protein synthesis can lead to conditions such as mitochondrial myopathy and MELAS syndrome (Mitochondrial Encephalopathy Lactic Acidosis and Stroke-like episodes). MRPS35 through its role in mitochondrial protein synthesis also has connections with proteins like MRPL44 which is another component of the mitochondrial ribosome where defects in these proteins can potentially exacerbate mitochondrial dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MRPS35

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:2563-2575 PubMed34130593

2021

Lysine Acetyltransferase 2B predicts favorable prognosis and functions as anti-oncogene in cervical carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Li,Juntao Zhang,Shuping Cao

Proteomics 21:e2000262 PubMed33763969

2021

Proteomics reveals the potential mechanism of Mrps35 controlling Listeria monocytogenes intracellular proliferation in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangbei Yuan,Zhangfu Li,Fang Li,Zewei Lin,Siyu Yao,Hang Zhou,Wenhu Liu,Haili Yu,Yang Liu,Fang Liu,Fei Li,Haiying Ran,Junying Zhang,Yi Huang,Qihuan Fu,Liting Wang,Jikui Liu

Cell metabolism 33:1027-1041.e8 PubMed33770508

2021

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ping Wang,Azeem Sharda,Shuang-Nian Xu,Nick van Gastel,Cheuk Him Man,Una Choi,Wei Zhong Leong,Xi Li,David T Scadden

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MRPS35 antibody [EPR11731(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] (AB182160)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] (AB182160)

Western blot - Anti-MRPS35 antibody [EPR11731(2)] (AB182160)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise