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AB250602

Anti-MRPS35 antibody [EPR11731(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MRPS35 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MRPS28, HDCMD11P, MDS023, PSEC0213, MRPS35, Small ribosomal subunit protein mS35, MRP-S28, S28mt, MRP-S35, S35mt

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] - BSA and Azide free (AB250602)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] - BSA and Azide free (AB250602)

This data was developed using ab182160, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MRPS35 with ab182160 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] - BSA and Azide free (AB250602)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS35 antibody [EPR11731(2)] - BSA and Azide free (AB250602)

This data was developed using ab182160, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling MRPS35 with ab182160 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-MRPS35 antibody [EPR11731(2)] - BSA and Azide free (AB250602)
  • WB

Supplier Data

Western blot - Anti-MRPS35 antibody [EPR11731(2)] - BSA and Azide free (AB250602)

This data was developed using ab182160, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPS35 antibody [EPR11731(2)] (<a href='/en-us/products/primary-antibodies/mrps35-antibody-epr117312-ab182160'>ab182160</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11731(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

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Anti-MRPL12 antibody

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB191891

Anti-MRPS18B antibody [EPR19298]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250602 is the carrier-free version of ab182160.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRPS35 also known as 28S ribosomal protein S35 or mitochondrial ribosomal protein S35 is a component of the small subunit of the mitochondrial ribosome. This protein is integral to mitochondrial protein synthesis and weighs approximately 39 kDa. It is expressed widely in human tissues but shows higher expression in tissues with high energy demand like skeletal muscle and heart. MRPS35 plays a mechanical role in facilitating the translation of mitochondrial mRNA to proteins necessary for mitochondrial function.
Biological function summary

MRPS35 is essential in mitochondrial ribosome assembly and protein synthesis. As part of the mitochondrial ribosomal small subunit it contributes to the assembly of ribosomes and the translation of essential proteins for mitochondrial energy production. This protein partners with other mitochondrial ribosomal proteins and plays a role in the synthesis of oxidative phosphorylation components an important metabolic pathway for ATP production.

Pathways

MRPS35 is significant in cellular respiratory processes. It plays a role in the oxidative phosphorylation pathway which is important for ATP production. MRPS35 interacts with other mitochondrial ribosomal proteins like MRPS30 to ensure efficient assembly and function of the mitochondrial ribosome. The proper synthesis of mitochondrial proteins is critical for maintaining the electron transport chain's function.

MRPS35 relates to certain mitochondrial disorders and possibly metabolic syndromes. Defects in mitochondrial protein synthesis can lead to conditions such as mitochondrial myopathy and MELAS syndrome (Mitochondrial Encephalopathy Lactic Acidosis and Stroke-like episodes). MRPS35 through its role in mitochondrial protein synthesis also has connections with proteins like MRPL44 which is another component of the mitochondrial ribosome where defects in these proteins can potentially exacerbate mitochondrial dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPS35
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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