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AB224442

Anti-MRPS7 antibody

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(2 Publications)

Rabbit Polyclonal MRPS7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MRPS7 aa 50-200.

View Alternative Names

Small ribosomal subunit protein uS7m, bMRP-27a, MRP-S7, S7mt, bMRP27a, MRPS7

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS7 antibody (AB224442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS7 antibody (AB224442)

Paraffin-embedded human liver tissue stained for MRPS7 using ab224442 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MRPS7 antibody (AB224442)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRPS7 antibody (AB224442)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MRPS7 (green) using ab224442 at 4 μg/ml in ICC/IF.

Western blot - Anti-MRPS7 antibody (AB224442)
  • WB

Supplier Data

Western blot - Anti-MRPS7 antibody (AB224442)

All lanes:

Western blot - Anti-MRPS7 antibody (ab224442) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MRPS7 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2R9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MRPS7 protein also known as mitochondrial ribosomal protein S7 plays a major role in protein synthesis within mitochondria. The mass of MRPS7 is approximately 20 kDa. The protein is expressed within the mitochondria of cells. It is a component of the small 28S subunit of the mitochondrial ribosome which is distinct from the cytoplasmic ribosomes. MRPS7 is an essential part of the ribosome's machinery ensuring the accurate translation of mitochondrial genes into functional proteins.
Biological function summary

MRPS7 is involved in mitochondrial translation a process critical for cellular respiration and energy production. This protein is part of the mitochondrial ribosomal complex which includes both proteins and rRNA. By playing an active role in assembling and stabilizing the ribosome MRPS7 helps in translating mRNA transcribed from mitochondrial DNA. This translation is necessary for the production of essential components of the electron transport chain which generates ATP.

Pathways

MRPS7 engages in the mitochondrial translation pathway which ensures the synthesis of proteins required for the oxidative phosphorylation pathway. It interacts with other mitochondrial ribosomal proteins like MRPS28 to form a functional ribosome. Through these interactions MRPS7 helps maintain the efficiency and fidelity of mitochondrial protein synthesis directly affecting cellular energy metabolism and homeostasis.

Research has associated defects in MRPS7 with disorders involving mitochondrial dysfunction such as mitochondrial myopathy. This condition impacts muscle function due to impaired energy production in the mitochondria. MRPS7 mutations may also relate to neurological disorders where mitochondrial performance is compromised. Such conditions often involve other mitochondrial ribosomal proteins like MRPL3 indicating a network of protein interactions necessary for mitochondrial integrity and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MRPS7
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of human genetics 112:59-74 PubMed39701103

2024

Bi-allelic variants in DAP3 result in reduced assembly of the mitoribosomal small subunit with altered apoptosis and a Perrault-syndrome-spectrum phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas B Smith,Robert Kopajtich,Leigh A M Demain,Alessandro Rea,Huw B Thomas,Manuel Schiff,Christian Beetz,Shelagh Joss,Gerard S Conway,Anju Shukla,Mayuri Yeole,Periyasamy Radhakrishnan,Hatem Azzouz,Amel Ben Chehida,Monique Elmaleh-Bergès,Ruth I C Glasgow,Kyle Thompson,Monika Oláhová,Langping He,Emma M Jenkinson,Amir Jahic,Inna A Belyantseva,Melanie Barzik,Jill E Urquhart,James O'Sullivan,Simon G Williams,Sanjeev S Bhaskar,Samantha Carrera,Alexander J M Blakes,Siddharth Banka,Wyatt W Yue,Jamie M Ellingford,Henry Houlden,Kevin J Munro,Thomas B Friedman,Robert W Taylor,Holger Prokisch,Raymond T O'Keefe,William G Newman

Scientific reports 13:20430 PubMed37993584

2023

NPLOC4 is a potential target and a poor prognostic signature in lung squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Naixue Wang,Dantong Zhu,Yao Liu,Jingran Wu,Meiling Wang,Shanxiu Jin,Fangwei Fu,Baolei Li,Hongjuan Ji,Cheng Du,Zhendong Zheng
View all publications
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