Rabbit Polyclonal MS2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ADAM8 aa 650 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ADAM8 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P78325
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Possible involvement in extravasation of leukocytes.
Alternative names
CD156a, MS2, ADAM8, Disintegrin and metalloproteinase domain-containing protein 8, ADAM 8, Cell surface antigen MS2
Recommended products
Rabbit Polyclonal MS2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ADAM8 aa 650 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ADAM8 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P78325
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-MS2 antibody (ab236949)
All lanes: Western blot - Anti-MS2 antibody (ab236949) at 1/500 dilution
Lane 1: Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2: THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 3: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 89 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody (ab236949)
Paraffin-embedded human lymph node tissue stained for MS2 using ab236949 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody (ab236949)
Paraffin-embedded human pancreas tissue stained for MS2 using ab236949 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunocytochemistry/ Immunofluorescence - Anti-MS2 antibody (ab236949)
MCF7 (human breast adenocarcinoma cell line) cells stained for MS2 (green) using ab236949 at 1/133 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI (Blue).
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