AB250593

Anti-MS4A14 antibody [EPR14020] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MS4A14 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

MS4A13, MS4A16, MS4A14, Membrane-spanning 4-domains subfamily A member 14, Testis development protein NYD-SP21

2 Images

Supplier Data

Western blot - Anti-MS4A14 antibody [EPR14020] - BSA and Azide free (AB250593)

This data was developed using ab182151, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MS4A14 antibody [EPR14020] (<a href='/en-us/products/primary-antibodies/ms4a14-antibody-epr14020-ab182151'>ab182151</a>) at 1/20000 dilution

Lane 1:

Human thymus lysate at 10 µg

Lane 2:

Human testis lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 122 kDa,77 kDa

false

Supplier Data

Western blot - Anti-MS4A14 antibody [EPR14020] - BSA and Azide free (AB250593)

This data was developed using ab182151, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MS4A14 antibody [EPR14020] (<a href='/en-us/products/primary-antibodies/ms4a14-antibody-epr14020-ab182151'>ab182151</a>) at 1/20000 dilution

Lane 1:

THP1 cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 77 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14020

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250593 is the carrier-free version of ab182151.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MS4A14 also known by its alternate names is part of the membrane-spanning 4-domains subfamily A. It has a molecular mass of approximately 54 kDa. This protein usually expresses in specific tissues such as the brain and testes. MS4A14 has a structure characterized by four transmembrane domains which indicates its role in cellular communication and membrane signaling processes.

Biological function summary

This protein participates in modulating signal transduction across the cellular membrane. MS4A14 associates with lipid rafts which facilitates its role in signaling events. It does not operate alone but as part of larger protein complexes interacting with other proteins to carry out its function. The precise complexes it forms are under continued research seeking to clarify its biological roles fully.

Pathways

This protein integrates within certain cellular signaling cascades. One important pathway it involves is the calcium-mediated signaling pathway. MS4A14 interacts with various proteins within this pathway such as calcium channels to help regulate ion flow across membranes. Another related pathway is immune response modulation though its exact partners in these cascades require further investigation to fully understand its integrative roles.

MS4A14 has links to Alzheimer's disease and certain forms of cancer. In Alzheimer's it interacts with proteins involved in amyloid-beta processing which plays an important role in disease progression. Its relation to cancer seems to connect to pathways influencing cell proliferation and apoptosis but the exact mechanisms and associated proteins are still subjects of active research. Understanding these connections could inform potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in signal transduction as a component of a multimeric receptor complex.

See full target information MS4A14

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MS4A14 antibody [EPR14020] - BSA and Azide free

Western blot - Anti-MS4A14 antibody [EPR14020] - BSA and Azide free (AB250593)

Western blot - Anti-MS4A14 antibody [EPR14020] - BSA and Azide free (AB250593)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise