Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (AB280570)

This data was developed using ab271068, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling MS4A2 with ab271068 at 1/2000 dilution (0.221 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mast cells of human breast carcinoma. The section was incubated with ab271068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (AB280570)

This data was developed using ab271068, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling MS4A2 with ab271068 at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) antibody at 1/500 dilution (Red).

Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged MS4A2 expression vector (PMID : 18571665).

ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor^® 488) was used to counterstain tubulin at 1/100 dilution (Green). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (AB280570)

This data was developed using ab271068, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labelling MS4A2 with ab271068 at 1/2000 dilution (0.221 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mast cells of human prostatic hyperplasia. The section was incubated with ab271068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (AB280570)

This data was developed using ab271068, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue labelling MS4A2 with ab271068 at 1/2000 dilution (0.221 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mast cells of human colon. The section was incubated with ab271068 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (AB280570)

This data was developed using ab271068, the same antibody clone in a different buffer formulation.

MS4A2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with MS4A2 expression vector, whole cell lysate 10 μg with ab271068 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271068 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293T transfected with MS4A2 expression vector, whole cell lysate 10 μg

Lane 2 : ab271068 IP in HEK-293T transfected with MS4A2 expression vector, whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab271068 in HEK-293T transfected with MS4A2 expression vector, whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 7.75 seconds

All lanes:

Immunoprecipitation - Anti-MS4A2 antibody [EPR24324-7] (<a href='/en-us/products/primary-antibodies/ms4a2-antibody-epr24324-7-ab271068'>ab271068</a>)

Predicted band size: 27 kDa

false

• Dot

Supplier Data

Dot Blot - Anti-MS4A2 antibody [EPR24324-7] - BSA and Azide free (AB280570)

This data was developed using ab271068, the same antibody clone in a different buffer formulation.

Dot blot analysis of MS4A2 using ab271068 at 1 : 1000 dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : Human MS4A2 peptide (aa 226-243)
Lane 2 : Human MS4A2 peptide (aa 206-223)
Lane 3 : Human MS4A2 peptide (aa 151-171)

Exposure time : 3 minutes

Blocking and diluting buffer and concentration : 5% NFDM/TBST