Skip to main content

Rabbit Recombinant Monoclonal MS4A4A antibody. Suitable for IP, IHC-P and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

May be involved in signal transduction as a component of a multimeric receptor complex.

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Recombinant Monoclonal MS4A4A antibody. Suitable for IP, IHC-P and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR24399-144

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

MS4A4A influences cellular communication and immune response. It is part of larger multiprotein complexes on the cell membrane helping mediate interactions between cells and extracellular signaling molecules. These interactions are important for triggering immune responses and maintaining tissue homeostasis. MS4A4A interacts with a range of cytokines which makes it important for modulating immune cell activities and facilitating immune surveillance.

Activity summary

MS4A4A also known as Membrane-spanning 4-domains subfamily A member 4A is a protein with a mass of approximately 25 kilodaltons. It is a member of the MS4A family which includes several proteins sharing similar structural features such as four transmembrane domains. MS4A4A is mainly expressed on the cell surface of immune cells including monocytes and macrophages. Its expression is notably observed in spleen and peripheral blood leukocytes suggesting an important role in the immune system.

Pathways

MS4A4A influences immune signaling pathways and impacts inflammation processes. It is involved in the NF-κB signaling pathway which is an important mediator of immune response inflammation and cell survival. MS4A4A also interacts with proteins like CD20 and other MS4A family members which play roles in cell cycle and apoptosis regulation. These interactions ensure effective signal transduction and immune regulation making it a significant participant in maintaining cellular homeostasis.

Associated diseases and disorders

MS4A4A has associations with inflammatory diseases and certain types of cancer. It is connected to the pathology of chronic inflammatory conditions where its overexpression may lead to excessive immune responses. MS4A4A is also implicated in hematologic malignancies where it might influence tumor growth and immune evasion. In such contexts it often interacts with proteins like CD20 which are essential for B-cell development and differentiation. Understanding MS4A4A's involvement in these disorders provides insights into potential therapeutic strategies targeting immune-related pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] (ab271069), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] (ab271069)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in immune cells of human colon (PMID: 31263276). The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] (ab271069), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] (ab271069)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in immune cells of human tonsil. The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] (ab271069), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] (ab271069)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human cerebrum (PMID: 23874341). The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-MS4A4A antibody [EPR24399-144] (ab271069), expandable thumbnail

    Immunoprecipitation - Anti-MS4A4A antibody [EPR24399-144] (ab271069)

    MS4A4A was immunoprecipitated from 0.35 mg Human placenta tissue lysate with ab271069 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271069 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human placenta tissue lysate 10 ug

    Lane 2: ab271069 IP in Human placenta tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271069 in human placenta tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-MS4A4A antibody [EPR24399-144] (AB271069)

    Predicted band size: 25 kDa

    Observed band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] (ab271069), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] (ab271069)

    Immunohistochemical analysis of paraffin-embedded Human glioma tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human glioma. The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com