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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
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Rabbit Recombinant Monoclonal MS4A4A antibody. Suitable for IP, IHC-P and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Not recommended | Tested | Not recommended | Tested | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
May be involved in signal transduction as a component of a multimeric receptor complex.
Membrane-spanning 4-domains subfamily A member 4A, CD20 antigen-like 1, Four-span transmembrane protein 1, MS4A4A, 4SPAN1, CD20L1, HDCME31P, MS4A4
Rabbit Recombinant Monoclonal MS4A4A antibody. Suitable for IP, IHC-P and reacts with Human samples.
Membrane-spanning 4-domains subfamily A member 4A, CD20 antigen-like 1, Four-span transmembrane protein 1, MS4A4A, 4SPAN1, CD20L1, HDCME31P, MS4A4
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR24399-144
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
MS4A4A influences cellular communication and immune response. It is part of larger multiprotein complexes on the cell membrane helping mediate interactions between cells and extracellular signaling molecules. These interactions are important for triggering immune responses and maintaining tissue homeostasis. MS4A4A interacts with a range of cytokines which makes it important for modulating immune cell activities and facilitating immune surveillance.
MS4A4A also known as Membrane-spanning 4-domains subfamily A member 4A is a protein with a mass of approximately 25 kilodaltons. It is a member of the MS4A family which includes several proteins sharing similar structural features such as four transmembrane domains. MS4A4A is mainly expressed on the cell surface of immune cells including monocytes and macrophages. Its expression is notably observed in spleen and peripheral blood leukocytes suggesting an important role in the immune system.
MS4A4A influences immune signaling pathways and impacts inflammation processes. It is involved in the NF-κB signaling pathway which is an important mediator of immune response inflammation and cell survival. MS4A4A also interacts with proteins like CD20 and other MS4A family members which play roles in cell cycle and apoptosis regulation. These interactions ensure effective signal transduction and immune regulation making it a significant participant in maintaining cellular homeostasis.
MS4A4A has associations with inflammatory diseases and certain types of cancer. It is connected to the pathology of chronic inflammatory conditions where its overexpression may lead to excessive immune responses. MS4A4A is also implicated in hematologic malignancies where it might influence tumor growth and immune evasion. In such contexts it often interacts with proteins like CD20 which are essential for B-cell development and differentiation. Understanding MS4A4A's involvement in these disorders provides insights into potential therapeutic strategies targeting immune-related pathways.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in immune cells of human colon (PMID: 31263276). The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in immune cells of human tonsil. The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human cerebrum (PMID: 23874341). The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
MS4A4A was immunoprecipitated from 0.35 mg Human placenta tissue lysate with ab271069 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271069 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human placenta tissue lysate 10 ug
Lane 2: ab271069 IP in Human placenta tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271069 in human placenta tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-MS4A4A antibody [EPR24399-144] (AB271069)
Predicted band size: 25 kDa
Observed band size: 23 kDa
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human glioma. The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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