AB281837

Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MS4A4A antibody. Carrier free. Suitable for IP, IHC-P and reacts with Human samples.

View Alternative Names

4SPAN1, CD20L1, MS4A4, HDCME31P, MS4A4A, Membrane-spanning 4-domains subfamily A member 4A, CD20 antigen-like 1, Four-span transmembrane protein 1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

This data was developed using ab271069, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in human cerebrum (PMID : 23874341). The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

This data was developed using ab271069, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in immune cells of human tonsil. The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

This data was developed using ab271069, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in immune cells of human colon (PMID : 31263276). The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

This data was developed using ab271069, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labelling MS4A4A with ab271069 at 1/2000 dilution (0.216 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in human glioma. The section was incubated with ab271069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Supplier Data

Immunoprecipitation - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

This data was developed using ab271069, the same antibody clone in a different buffer formulation.

MS4A4A was immunoprecipitated from 0.35 mg Human placenta tissue lysate with ab271069 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271069 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human placenta tissue lysate 10 ug

Lane 2 : ab271069 IP in Human placenta tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab271069 in human placenta tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-MS4A4A antibody [EPR24399-144] (<a href='/en-us/products/primary-antibodies/ms4a4a-antibody-epr24399-144-ab271069'>ab271069</a>)

Predicted band size: 25 kDa

Observed band size: 23 kDa

false

Unconjugated

Anti-MS4A4A antibody [EPR24399-144]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24399-144

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab281837 is the carrier-free version of ab271069.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MS4A4A also known as Membrane-spanning 4-domains subfamily A member 4A is a protein with a mass of approximately 25 kilodaltons. It is a member of the MS4A family which includes several proteins sharing similar structural features such as four transmembrane domains. MS4A4A is mainly expressed on the cell surface of immune cells including monocytes and macrophages. Its expression is notably observed in spleen and peripheral blood leukocytes suggesting an important role in the immune system.

Biological function summary

MS4A4A influences cellular communication and immune response. It is part of larger multiprotein complexes on the cell membrane helping mediate interactions between cells and extracellular signaling molecules. These interactions are important for triggering immune responses and maintaining tissue homeostasis. MS4A4A interacts with a range of cytokines which makes it important for modulating immune cell activities and facilitating immune surveillance.

Pathways

MS4A4A influences immune signaling pathways and impacts inflammation processes. It is involved in the NF-κB signaling pathway which is an important mediator of immune response inflammation and cell survival. MS4A4A also interacts with proteins like CD20 and other MS4A family members which play roles in cell cycle and apoptosis regulation. These interactions ensure effective signal transduction and immune regulation making it a significant participant in maintaining cellular homeostasis.

MS4A4A has associations with inflammatory diseases and certain types of cancer. It is connected to the pathology of chronic inflammatory conditions where its overexpression may lead to excessive immune responses. MS4A4A is also implicated in hematologic malignancies where it might influence tumor growth and immune evasion. In such contexts it often interacts with proteins like CD20 which are essential for B-cell development and differentiation. Understanding MS4A4A's involvement in these disorders provides insights into potential therapeutic strategies targeting immune-related pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in signal transduction as a component of a multimeric receptor complex.

See full target information MS4A4A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

Immunoprecipitation - Anti-MS4A4A antibody [EPR24399-144] - BSA and Azide free (AB281837)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise