Skip to main content

Rabbit Recombinant Monoclonal MS4A4A_MOUSE antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Transfected cell line samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (AB307356), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (AB307356), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (AB307356), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (AB307356), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (AB307356), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFIHC-FrIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Rat, Mouse, Human, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human, Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human, Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Transfected cell line
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat, Mouse, Human, Transfected cell line
Dilution info
-
Notes

-

Target data

Alternative names

4SPAN1, CD20 antigen like 1, CD20L1, MS4A4

Recommended products

Rabbit Recombinant Monoclonal MS4A4A_MOUSE antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Transfected cell line samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27242-39
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MS4A4A also known as Membrane-spanning 4-domains subfamily A member 4A is a protein with a mass of approximately 25 kilodaltons. It is a member of the MS4A family which includes several proteins sharing similar structural features such as four transmembrane domains. MS4A4A is mainly expressed on the cell surface of immune cells including monocytes and macrophages. Its expression is notably observed in spleen and peripheral blood leukocytes suggesting an important role in the immune system.

Biological function summary

MS4A4A influences cellular communication and immune response. It is part of larger multiprotein complexes on the cell membrane helping mediate interactions between cells and extracellular signaling molecules. These interactions are important for triggering immune responses and maintaining tissue homeostasis. MS4A4A interacts with a range of cytokines which makes it important for modulating immune cell activities and facilitating immune surveillance.

Pathways

MS4A4A influences immune signaling pathways and impacts inflammation processes. It is involved in the NF-κB signaling pathway which is an important mediator of immune response inflammation and cell survival. MS4A4A also interacts with proteins like CD20 and other MS4A family members which play roles in cell cycle and apoptosis regulation. These interactions ensure effective signal transduction and immune regulation making it a significant participant in maintaining cellular homeostasis.

Associated diseases and disorders

MS4A4A has associations with inflammatory diseases and certain types of cancer. It is connected to the pathology of chronic inflammatory conditions where its overexpression may lead to excessive immune responses. MS4A4A is also implicated in hematologic malignancies where it might influence tumor growth and immune evasion. In such contexts it often interacts with proteins like CD20 which are essential for B-cell development and differentiation. Understanding MS4A4A's involvement in these disorders provides insights into potential therapeutic strategies targeting immune-related pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356)

    This data was developed using Anti-MS4A4A antibody [EPR27242-39] ab307355, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse pancreatic tum tissue labeling MS4A4A with Anti-MS4A4A antibody [EPR27242-39] ab307355 at 1/500 (0.982 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on immune cells of mouse pancreatic tumor. The section was incubated with Anti-MS4A4A antibody [EPR27242-39] ab307355 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356)

    This data was developed using Anti-MS4A4A antibody [EPR27242-39] ab307355, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MS4A4A with Anti-MS4A4A antibody [EPR27242-39] ab307355 at 1/500 (0.982 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on immune cells of mouse colon. The section was incubated with Anti-MS4A4A antibody [EPR27242-39] ab307355 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356)

    This data was developed using Anti-MS4A4A antibody [EPR27242-39] ab307355, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MS4A4A with Anti-MS4A4A antibody [EPR27242-39] ab307355 at 1/500 (0.982 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: no staining on mouse cerebrum (PMID:11245982). The section was incubated with Anti-MS4A4A antibody [EPR27242-39] ab307355 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356)

    This data was developed using Anti-MS4A4A antibody [EPR27242-39] ab307355, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse colon (fresh) tissue lABeling MS4A4A with Anti-MS4A4A antibody [EPR27242-39] ab307355 at 1/100 (4.91 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse colon. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MS4A4A antibody [EPR27242-39] ab307355 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356)

    This data was developed using Anti-MS4A4A antibody [EPR27242-39] ab307355, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse cerebrum (fresh) tissue lABeling MS4A4A with Anti-MS4A4A antibody [EPR27242-39] ab307355 at 1/100 (4.91 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse cerebrum (PMID: 23874341). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MS4A4A antibody [EPR27242-39] ab307355 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS4A4A antibody [EPR27242-39] - BSA and Azide free (ab307356)

    This data was developed using Anti-MS4A4A antibody [EPR27242-39] ab307355, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling MS4A4A with Anti-MS4A4A antibody [EPR27242-39] ab307355 at 1/500 (0.982 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on HEK-293T transfected with a His-tagged Ms4a4a construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with Anti-MS4A4A antibody [EPR27242-39] ab307355 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com