Anti-MS4A4A antibody [EPR27243-59]

• WB

Supplier Data

Western blot - Anti-MS4A4A antibody [EPR27243-59] (AB319033)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the bands higher than 20 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MS4A4A antibody [EPR27243-59] (ab319033) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 50 µg

Lane 2:

Human colon tissue lysate at 50 µg

Lane 3:

Human tonsil tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution

Observed band size: 20 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-MS4A4A antibody [EPR27243-59] (AB319033)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : 293T

The expression of MS4A4A is upregulated in response to PMA treatment (PMID 28303902).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 180 seconds, lanes 3-4 : 48 seconds

All lanes:

Western blot - Anti-MS4A4A antibody [EPR27243-59] (ab319033) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 50 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

Lane 3:

Untreated U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 50 µg

Lane 4:

U-937 treated with 200 nM PMA for 3 days whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,36 kDa

false