Rabbit Recombinant Monoclonal MS4A4A antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-MS4A4A antibody [EPR27243-59] - BSA and Azide free (AB319034), expandable thumbnail](https://content.abcam.com/products/images/ms4a4a-antibody-epr27243-59-bsa-and-azide-free-ab319034--western-blot-img434412.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MS4A4A antibody [EPR27243-59] - BSA and Azide free (AB319034)")
![Western blot - Anti-MS4A4A antibody [EPR27243-59] - BSA and Azide free (AB319034), expandable thumbnail](https://content.abcam.com/products/images/ms4a4a-antibody-epr27243-59-bsa-and-azide-free-ab319034--western-blot-img434413.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MS4A4A antibody [EPR27243-59] - BSA and Azide free (AB319034)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
May be involved in signal transduction as a component of a multimeric receptor complex.
Alternative names
4SPAN1, CD20L1, MS4A4, HDCME31P, MS4A4A, Membrane-spanning 4-domains subfamily A member 4A, CD20 antigen-like 1, Four-span transmembrane protein 1
Recommended products
Rabbit Recombinant Monoclonal MS4A4A antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27243-59
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab319034 is the carrier-free version of Anti-MS4A4A antibody [EPR27243-59] ab319033.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MS4A4A also known as Membrane-spanning 4-domains subfamily A member 4A is a protein with a mass of approximately 25 kilodaltons. It is a member of the MS4A family which includes several proteins sharing similar structural features such as four transmembrane domains. MS4A4A is mainly expressed on the cell surface of immune cells including monocytes and macrophages. Its expression is notably observed in spleen and peripheral blood leukocytes suggesting an important role in the immune system.
- Biological function summary
MS4A4A influences cellular communication and immune response. It is part of larger multiprotein complexes on the cell membrane helping mediate interactions between cells and extracellular signaling molecules. These interactions are important for triggering immune responses and maintaining tissue homeostasis. MS4A4A interacts with a range of cytokines which makes it important for modulating immune cell activities and facilitating immune surveillance.
- Pathways
MS4A4A influences immune signaling pathways and impacts inflammation processes. It is involved in the NF-κB signaling pathway which is an important mediator of immune response inflammation and cell survival. MS4A4A also interacts with proteins like CD20 and other MS4A family members which play roles in cell cycle and apoptosis regulation. These interactions ensure effective signal transduction and immune regulation making it a significant participant in maintaining cellular homeostasis.
- Associated diseases and disorders
MS4A4A has associations with inflammatory diseases and certain types of cancer. It is connected to the pathology of chronic inflammatory conditions where its overexpression may lead to excessive immune responses. MS4A4A is also implicated in hematologic malignancies where it might influence tumor growth and immune evasion. In such contexts it often interacts with proteins like CD20 which are essential for B-cell development and differentiation. Understanding MS4A4A's involvement in these disorders provides insights into potential therapeutic strategies targeting immune-related pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-MS4A4A antibody [EPR27243-59] - BSA and Azide free (ab319034), expandable thumbnail](https://content.abcam.com/products/images/ms4a4a-antibody-epr27243-59-bsa-and-azide-free-ab319034--western-blot-img434412.jpg "Western blot - Anti-MS4A4A antibody [EPR27243-59] - BSA and Azide free (ab319034)")
Western blot - Anti-MS4A4A antibody [EPR27243-59] - BSA and Azide free (ab319034)
This data was developed using Anti-MS4A4A antibody [EPR27243-59] ab319033, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: 293T
The expression of MS4A4A is upregulated in response to PMA treatment (PMID 28303902).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2:180 seconds, lanes 3-4: 48 seconds
All lanes: Western blot - Anti-MS4A4A antibody [EPR27243-59] (Anti-MS4A4A antibody [EPR27243-59] ab319033) at 1/1000 dilution
Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 50 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 50 µg
Lane 3: Untreated U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 50 µg
Lane 4: U-937 treated with 200 nM PMA for 3 days whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 20 kDa, 36 kDa
![Western blot - Anti-MS4A4A antibody [EPR27243-59] - BSA and Azide free (ab319034), expandable thumbnail](https://content.abcam.com/products/images/ms4a4a-antibody-epr27243-59-bsa-and-azide-free-ab319034--western-blot-img434413.jpg "Western blot - Anti-MS4A4A antibody [EPR27243-59] - BSA and Azide free (ab319034)")
Western blot - Anti-MS4A4A antibody [EPR27243-59] - BSA and Azide free (ab319034)
This data was developed using Anti-MS4A4A antibody [EPR27243-59] ab319033, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregation.
The identity of the bands higher than 20 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MS4A4A antibody [EPR27243-59] (Anti-MS4A4A antibody [EPR27243-59] ab319033) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 50 µg
Lane 2: Human colon tissue lysate at 50 µg
Lane 3: Human tonsil tissue lysate at 50 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
Observed band size: 20 kDa, 36 kDa
Exposure time: 180s
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