AB221097

Anti-MSC antibody

Name: Anti-MSC antibody (ab221097) | Abcam
Brand: Abcam Limited
SKU: AB221097

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(2 Publications)

Rabbit Polyclonal MSC antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MSC aa 1-50.

View Alternative Names

ABF1, BHLHA22, MSC, Musculin, Activated B-cell factor 1, Class A basic helix-loop-helix protein 22, ABF-1, bHLHa22

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MSC antibody (AB221097)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MSC antibody (AB221097)

Immunofluorescent analysis of paraformaldehyde-fixed, Triton X-100 permeabilized SK-MEL-30 cells labeling MSC with ab221097 at 4 μg/mL dilution (green).

Supplier Data

Western blot - Anti-MSC antibody (AB221097)

All lanes:

Western blot - Anti-MSC antibody (ab221097) at 1/100 dilution

All lanes:

RT-4 cell lysate

Predicted band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MSC aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O60682

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target MSC short for Mesenchymal Stem Cell is a multipotent stromal cell capable of differentiating into a variety of cell types including osteoblasts chondrocytes and adipocytes. MSCs usually express surface markers such as CD73 CD90 and CD105 while lacking expression of hematopoietic markers like CD45 and CD34. These cells are primarily found in the bone marrow but are also present in adipose tissue umbilical cord blood and other tissues. MSCs typically range in size from 15 to 30 µm and play a significant role in tissue homeostasis and repair.

Biological function summary

MSCs engage in critical roles concerning tissue regeneration and immune modulation. MSCs interact within a complex cellular microenvironment to promote healing by secreting a variety of cytokines and growth factors. These cells aid in the down-regulation of immune responses which is vital for transplantation tolerance and autoimmunity treatment. While they are not strictly part of a single cellular complex their interactions with immune cells such as T-cells and dendritic cells highlight their multifaceted functionality.

Pathways

Mesenchymal Stem Cells participate in the Wnt/β-catenin and TGF-β signaling pathways. In the Wnt/β-catenin pathway MSCs influence cell proliferation and differentiation engaging proteins such as β-catenin and glycogen synthase kinase 3β (GSK-3β). In the context of the TGF-β pathway MSCs are involved in the regulation of extracellular matrix production and maintenance interacting with proteins like SMAD2/3 to convey signals essential for cellular processes. These pathways underline MSCs' involvement in both regenerative and regulatory roles in various tissue contexts.

MSCs show promise in treating osteoarthritis and graft-versus-host disease due to their regenerative and immunomodulatory properties. In osteoarthritis MSCs contribute to cartilage repair and anti-inflammatory modulation working in conjunction with proteins like matrix metalloproteinases (MMPs) to restore joint function. For graft-versus-host disease MSCs help mitigate immune reactions via interactions with T-cell receptors creating an environment conducive to tissue repair and immune tolerance. These associations highlight MSCs as potential therapeutic agents in regenerative medicine.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription repressor capable of inhibiting the transactivation capability of TCF3/E47. May play a role in regulating antigen-dependent B-cell differentiation.

See full target information MSC

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e40405 PubMed39687103

2024

Establishment of prognostic risk model related to disulfidptosis and immune infiltration in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Xu,Chong Pang,Xundi Xu

Clinical genetics 94:575-580 PubMed30221343

2018

Genomic duplication in the 19q13.42 imprinted region identified as a new genetic cause of intrauterine growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Graciane Petre,Patrick Lorès,Hervé Sartelet,Aurélie Truffot,Brice Poreau,Sandrine Brandeis,Guillaume Martinez,Véronique Satre,Radu Harbuz,Pierre F Ray,Florence Amblard,Françoise Devillard,Gaëlle Vieville,Francois Berger,Pierre-Simon Jouk,Daniel Vaiman,Aminata Touré,Charles Coutton,Marie Bidart

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MSC antibody

Immunocytochemistry/ Immunofluorescence - Anti-MSC antibody (AB221097)

Western blot - Anti-MSC antibody (AB221097)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise