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Mouse Monoclonal MSH2 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 31 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MSH2.


Images

Western blot - Anti-MSH2 antibody [3A2B8C] (AB52266), expandable thumbnail
  • Flow Cytometry - Anti-MSH2 antibody [3A2B8C] (AB52266), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [3A2B8C] (AB52266), expandable thumbnail
  • Immunoprecipitation - Anti-MSH2 antibody [3A2B8C] (AB52266), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [3A2B8C] (AB52266), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human MSH2. Database link P43246

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/2000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/1000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/1000.00000

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Alternative names

DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2

Recommended products

Mouse Monoclonal MSH2 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 31 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MSH2.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human MSH2. Database link P43246
Clone number

3A2B8C

Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.

This product was changed from ascites to supernatant. Lot no's high than GR128648-25 are from Tissue Culture Supernatant

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MSH2 also known as MutS Homolog 2 is a human protein with a molecular weight of approximately 100 kDa. It is an important component of the DNA mismatch repair system and plays an essential role in maintaining genomic stability by recognizing and repairing mismatched nucleotides during DNA replication. Expression of the MSH2 protein occurs broadly in dividing cells across various tissues with notable presence in tissues with high proliferation rates such as the colon and the endometrium. Additionally detection and quantification of MSH2 are often performed using methodologies such as MSH2 ELISA which aids in the assessment of its expression levels in different biological samples.

Biological function summary

Components are identified in mismatch repair where MSH2 forms a heterodimer with MSH6 known as the MutSα complex or with MSH3 known as the MutSβ complex. This heterodimerization is critical for the initial steps in the recognition and binding of mismatch errors on the DNA strand. MSH2 complex formation enables it to scan the DNA for errors facilitating the recruitment of additional repair proteins. The activity of MSH2 in these complexes is important in preserving the fidelity of genetic information and prevents mutations that could lead to genomic instability.

Pathways

MSH2 operates within the DNA damage response and repair pathways. The protein is a core component of the mismatch repair pathway which corrects DNA replication errors that elude proofreading activity of DNA polymerases. It interacts with other proteins such as MLH1 and PMS2 forming a synergistic function that amplifies the capacity to recognize and initiate repair of mismatches. The pathway involving MSH2 not only repairs mismatched bases but also plays a role in cell cycle control checkpoints and apoptosis evidencing its pivotal role in maintaining cell cycle integrity.

Associated diseases and disorders

Studies show that MSH2 is strongly associated with Lynch syndrome an autosomal dominant inherited condition that increases the risk of colorectal cancer. Mutations in the MSH2 gene impair its mismatch repair function and lead to microsatellite instability a hallmark of cancer cells in this disorder. Furthermore alterations in the MSH2 protein also relate to glioblastomas with correlations observed between MSH2 expression levels and tumor progression. These conditions exemplify the important role of MSH2 and its interaction with other DNA repair proteins in preventing cancerous developments.

Product promise

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6 product images

  • Western blot - Anti-MSH2 antibody [3A2B8C] (ab52266), expandable thumbnail

    Western blot - Anti-MSH2 antibody [3A2B8C] (ab52266)

    All lanes: Western blot - Anti-MSH2 antibody [3A2B8C] (ab52266) at 1/2000 dilution

    Lane 1: Cell lysates prepared from human Hela cells at 100 µg

    Lane 2: Cell lysates prepared from A549 cells at 100 µg

    Lane 3: Cell lysates prepared from human A431 cells at 100 µg

    Lane 4: Cell lysates prepared from HEK293 cells at 100 µg

    Secondary

    All lanes: HRP-conjugated Goat polyclonal to mouse IgG

    Predicted band size: 105 kDa

  • Flow Cytometry - Anti-MSH2 antibody [3A2B8C] (ab52266), expandable thumbnail

    Flow Cytometry - Anti-MSH2 antibody [3A2B8C] (ab52266)

    Overlay histogram showing HeLa cells stained with ab52266 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52266, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [3A2B8C] (ab52266), expandable thumbnail
    This image is courtesy of a customer review submitted by Dr Kirk McManus

    Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [3A2B8C] (ab52266)

    ab52266 (1/200) detecting MSH2 in HeLa cells (green). Cells were fixed in methanol (-20'C, 10min) and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.

  • Immunoprecipitation - Anti-MSH2 antibody [3A2B8C] (ab52266), expandable thumbnail

    Immunoprecipitation - Anti-MSH2 antibody [3A2B8C] (ab52266)

    MSH2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Mouse monoclonal to MSH2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52266.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/10,000 dilution.
    Band: 105kDa; MSH2

    All lanes: Immunoprecipitation - Anti-MSH2 antibody [3A2B8C] (ab52266)

    Predicted band size: 105 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [3A2B8C] (ab52266), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [3A2B8C] (ab52266)

    ab52266 at 1/1000 dilution staining MSH2 in human Hela cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. The primary antibody shows green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 phalloidin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [3A2B8C] (ab52266), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [3A2B8C] (ab52266)

    Immunohistochemical analysis of paraffin-embedded human rectum carcinoma tissue, showing nuclear and cytoplasmic localisation, using ab52266 at a dilution of 1/200 - 1/1000 with DAB staining.

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Product protocols

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