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AB70270

Anti-MSH2 antibody

5

(4 Reviews)

|

(28 Publications)

Rabbit Polyclonal MSH2 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Mouse, Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human MSH2 aa 850 to C-terminus.

View Alternative Names

DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody (AB70270)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody (AB70270)

ICC/IF image of ab70270 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70270, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody (AB70270)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody (AB70270)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node (left) and mouse squamous cell carcinoma (right) tissues labelling MSH2 with ab70270 at 1/1000 (1µg/ml) and 1/5000 (0.2µg/ml). Detection : DAB.

Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody (AB70270)
  • ICC/IF

AbReview45364****

Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody (AB70270)

ab70270 staining MSH2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

This image is courtesy of an Abreview submitted by Kirk McManus

Immunoprecipitation - Anti-MSH2 antibody (AB70270)
  • IP

Unknown

Immunoprecipitation - Anti-MSH2 antibody (AB70270)

Immunoprecipitation of HeLa whole cell lysate. Lane 1 : 50µg of input lysate. Lane 2 : HeLa whole cell lysate (1mg) immunoprecipitated with ab70270 at 3µg/mg. Lane 3 : HeLa whole cell lysate immunoprecipitated with control IgG. Samples were subjected to Western blot, analysed with ab70270 at 0.1µg/ml and detected by chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-MSH2 antibody (ab70270)

Predicted band size: 105 kDa

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Western blot - Anti-MSH2 antibody (AB70270)
  • WB

Unknown

Western blot - Anti-MSH2 antibody (AB70270)

All lanes:

Western blot - Anti-MSH2 antibody (ab70270) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

Ramos whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 105 kDa

Observed band size: 116 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB, IP

applications

Immunogen

Synthetic Peptide within Human MSH2 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P43246

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/15000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MSH2 also known as MutS Homolog 2 is a human protein with a molecular weight of approximately 100 kDa. It is an important component of the DNA mismatch repair system and plays an essential role in maintaining genomic stability by recognizing and repairing mismatched nucleotides during DNA replication. Expression of the MSH2 protein occurs broadly in dividing cells across various tissues with notable presence in tissues with high proliferation rates such as the colon and the endometrium. Additionally detection and quantification of MSH2 are often performed using methodologies such as MSH2 ELISA which aids in the assessment of its expression levels in different biological samples.
Biological function summary

Components are identified in mismatch repair where MSH2 forms a heterodimer with MSH6 known as the MutSα complex or with MSH3 known as the MutSβ complex. This heterodimerization is critical for the initial steps in the recognition and binding of mismatch errors on the DNA strand. MSH2 complex formation enables it to scan the DNA for errors facilitating the recruitment of additional repair proteins. The activity of MSH2 in these complexes is important in preserving the fidelity of genetic information and prevents mutations that could lead to genomic instability.

Pathways

MSH2 operates within the DNA damage response and repair pathways. The protein is a core component of the mismatch repair pathway which corrects DNA replication errors that elude proofreading activity of DNA polymerases. It interacts with other proteins such as MLH1 and PMS2 forming a synergistic function that amplifies the capacity to recognize and initiate repair of mismatches. The pathway involving MSH2 not only repairs mismatched bases but also plays a role in cell cycle control checkpoints and apoptosis evidencing its pivotal role in maintaining cell cycle integrity.

Studies show that MSH2 is strongly associated with Lynch syndrome an autosomal dominant inherited condition that increases the risk of colorectal cancer. Mutations in the MSH2 gene impair its mismatch repair function and lead to microsatellite instability a hallmark of cancer cells in this disorder. Furthermore alterations in the MSH2 protein also relate to glioblastomas with correlations observed between MSH2 expression levels and tumor progression. These conditions exemplify the important role of MSH2 and its interaction with other DNA repair proteins in preventing cancerous developments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers : MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed : 26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch : mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
See full target information MSH2
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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Nature aging 5:1739-1749 PubMed40836125

2025

Induced somatic mutation accumulation during skeletal muscle regeneration reduces muscle strength.

Applications

Unspecified application

Species

Unspecified reactive species

Peter Vrtačnik,Lara G Merino,Santhilal Subhash,Hafdís T Helgadóttir,Matthieu Bardin,Fabiana Stefani,Depin Wang,Ping Chen,Irene Franco,Gwladys Revêchon,Maria Eriksson

Epigenetics & chromatin 18:24 PubMed40296143

2025

MSH2 is not required for either maintenance of DNA methylation or repeat contraction at the FMR1 locus in fragile X syndrome or the FXN locus in Friedreich's ataxia.

Applications

Unspecified application

Species

Unspecified reactive species

Jessalyn Grant-Bier,Kathryn Ruppert,Bruce Hayward,Karen Usdin,Daman Kumari

Neoplasia (New York, N.Y.) 62:101145 PubMed39985912

2025

Effect of MisMatch repair deficiency on metastasis occurrence in a syngeneic mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre Laplante,Reginaldo Rosa,Laetitia Nebot-Bral,Jordane Goulas,Caroline Pouvelle,Sergey Nikolaev,Aymeric Silvin,Patricia L Kannouche

Nature communications 15:8338 PubMed39333076

2024

Proteogenomic characterization of skull-base chordoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qilin Zhang,Ziyan Xu,Rui Han,Yunzhi Wang,Zhen Ye,Jiajun Zhu,Yixin Cai,Fan Zhang,Jiangyan Zhao,Boyuan Yao,Zhaoyu Qin,Nidan Qiao,Ruofan Huang,Jinwen Feng,Yongfei Wang,Wenting Rui,Fuchu He,Yao Zhao,Chen Ding

Cancer cell international 24:25 PubMed38200495

2024

DNA mismatch repair system regulates the expression of PD-L1 through DNMTs in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Guo,Ruijiao Lu,Weina Kong,Miyessar Anwar,Yangchun Feng

Disease models & mechanisms 16: PubMed37395714

2023

Acetaldehyde and defective mismatch repair increase colonic tumours in a Lynch syndrome model with Aldh1b1 inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Guia Cerretelli,Ying Zhou,Mike F Müller,David J Adams,Mark J Arends

Journal for immunotherapy of cancer 11: PubMed37072347

2023

BRD4 inhibition impairs DNA mismatch repair, induces mismatch repair mutation signatures and creates therapeutic vulnerability to immune checkpoint blockade in MMR-proficient tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Fu,Bin Yang,Yaoyuan Cui,Xingyuan Hu,Xi Li,Funian Lu,Tianyu Qin,Li Zhang,Zhe Hu,Ensong Guo,Junpeng Fan,Rourou Xiao,Wenting Li,Xu Qin,Dianxing Hu,Wenju Peng,Jingbo Liu,Beibei Wang,Gordon B Mills,Gang Chen,Chaoyang Sun

Science immunology 8:eade1167 PubMed36961908

2023

DNA repair mechanisms that promote insertion-deletion events during immunoglobulin gene diversification.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Hao,Chuanzong Zhan,Chaoyang Lian,Simin Luo,Wenyi Cao,Binbin Wang,Xia Xie,Xiaofei Ye,Tuantuan Gui,Claudia Voena,Chiara Pighi,Yanyan Wang,Ying Tian,Xin Wang,Pengfei Dai,Yanni Cai,Xiaojing Liu,Shengqun Ouyang,Shiqi Sun,Qianwen Hu,Jun Liu,Youqiong Ye,Jingkun Zhao,Aiguo Lu,Ji-Yang Wang,Chuanxin Huang,Bing Su,Fei-Long Meng,Roberto Chiarle,Qiang Pan-Hammarström,Leng-Siew Yeap

Science advances 9:eadd8564 PubMed36921054

2023

Synthetic enforcement of STING signaling in cancer cells appropriates the immune microenvironment for checkpoint inhibitor therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Larsen Vornholz,Sophie E Isay,Zsuzsanna Kurgyis,Daniel C Strobl,Patricia Loll,Mohammed H Mosa,Malte D Luecken,Michael Sterr,Heiko Lickert,Christof Winter,Florian R Greten,Henner F Farin,Fabian J Theis,Jürgen Ruland

Journal of immunology (Baltimore, Md. : 1950) 210:369-376 PubMed36603026

2023

Ataxia Telangiectasia Mutated and MSH2 Control Blunt DNA End Joining in Ig Class Switch Recombination.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Sible,Mary Attaway,Giuseppe Fiorica,Genesis Michel,Jayanta Chaudhuri,Bao Q Vuong
View all publications
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