Anti-MSH2 antibody [EPR21017-2]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [EPR21017-2] (AB212188)

Immunofluorescent analysis of 100% methanol-fixed A-375 (human malignant melanoma cell line) cells labeling MSH2 with ab212188 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining in A-375 cell line is shown.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MSH2 antibody [EPR21017-2] (AB212188)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A-375 (humanmalignant melanoma cell line) cell line labeling MSH2 with ab212188 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MSH2 antibody [EPR21017-2] (AB212188)

MSH2 KO HAP1 (MSH2 knockout Human chronic myelogenous leukemia near-haploid cell line, Left) / WT HAP1 (Right) cells were fixed with4% paraformaldehyde and permeabilised with 90% methanol. The cells were incubated with the primary antibody, ab212188 at a 1/6000 dilution (0.01μg) (red line). The secondary antibody used wasGoat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at a 1/2000 dilution. The isotype control (black line) antibody wasRabbit monoclonal IgG (ab172730). An unlabelled control without incubation with primary antibody and secondary antibody was also performed (blue line).

90% methanol permeabilisation is recommended. Avoid using TritonX-100 as it may cause non-specific staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (AB212188)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining only in the stromal cells of human colon cancer; no staining observed in the tumor cells (PMID : 24710284). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (AB212188)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human testis was observed (PMID : 10029069). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (AB212188)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse testis was observed (PMID : 10029069). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (AB212188)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining was mainly observed in the spermatogonia in rat testis (PMID : 10029069). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• WB

Supplier Data

Western blot - Anti-MSH2 antibody [EPR21017-2] (AB212188)

Blocking/Dilution buffer : 5% NFDM/TBST.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-MSH2 antibody [EPR21017-2] (ab212188) at 1/1000 dilution

Lane 1:

Rat testis lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 3:

Human tonsil lysate at 10 µg

Lane 4:

A-375 (human malignant melanoma cell line) whole cell lysate at 10 µg

Lane 5:

C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Lane 6:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 7:

Human fetal heart lysate at 10 µg

Lane 8:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 105 kDa

Observed band size: 104 kDa

true

Exposure time: 3min